论文信息 - A Cleavable Biotinylated Photoactivable Derivative of α‐MSH: Its Application to the Characterization and Isolation of the α‐MSH Receptor a

A Cleavable Biotinylated Photoactivable Derivative of α‐MSH: Its Application to the Characterization and Isolation of the α‐MSH Receptor a

The presence of a-melanocyte-stimulating hormone (a-MSH) receptors on various mouse and human melanoma cell lines has been demonstrated by photoaffinity labeling.I.* The objective of this investigation was to develop an efficient method for the characterization and purification of the a-MSH receptor from B16 munne melanoma cells. The method described comprises three elements: (a) The synthesis of a cleavable biotinyiated photoactivable derivative of a-MSH. (b) The application of the streptavidin-biotin affinity system. (c) The use of magnetic beads as an immobilized support in place of conventional affinity columns.

[1] A. Eberle,et al. The receptor for alpha-melanotropin of mouse and human melanoma cells. Application of a potent alpha-melanotropin photoaffinity label. , 1989, The Journal of biological chemistry.

[2] A. Eberle,et al. Photoaffinity labelling of melanoma cell MSH receptors , 1987, FEBS letters.

[3] M. Nassal. 4‐(1‐Azi‐2,2,2‐trifluoroethyl)benzoic Acid, a Highly Photolabile Carbene Generating Label Readily Fixable to Biochemical Agents , 1983 .

[4] V. Hruby,et al. 4-Norleucine, 7-D-phenylalanine-alpha-melanocyte-stimulating hormone: a highly potent alpha-melanotropin with ultralong biological activity. , 1980, Proceedings of the National Academy of Sciences of the United States of America.