Importance of cholinesterase kinetic parameters in environmental monitoring using estuarine fish.

The aim of the present study was to determine the kinetic parameters and cholinesterase (ChE) activity in two teleost fish, the white mouth croaker Micropogonias furnieri (Scianidae) and the Madamango sea catfish Cathorops spixii (Ariidae), to verify their suitability as sentinel species of aquatic pollution by anticholinergic compounds. Individuals of each species were captured in one reference and one polluted site in the Southern Brazilian coast. Brain tissue was used as enzyme source. Inhibition kinetic parameters indicated that ChE from C. spixii collected at the reference site showed more affinity (Ka) for eserine that from those collected at the polluted site. The opposite was observed for the carbamylation constants (Kc). Thus, similar inhibition constants (Ki) were observed. M. furnieri brain showed an extremely low sensitivity to in vitro inhibition by eserine, indicating that it is not a suitable biomarker to be employed in environmental monitoring of anticholinergic compounds. Results from the present study also point to the need for considering kinetic analysis when cholinesterase activity is employed as a biomarker.

[1]  G. Rodríguez‐Fuentes,et al.  Characterization of cholinesterase activity from different tissues of Nile tilapia (Oreochromis niloticus). , 2004, Marine environmental research.

[2]  V. Cassano,et al.  Methyl-paraoxon comparative inhibition kinetics for acetylcholinesterases from brain of neotropical fishes. , 2004, Toxicology letters.

[3]  J. Narbonne,et al.  Cholinesterases from the marine mussels Mytilus galloprovincialis Lmk. and M. edulis L. from the freshwater bivalve Corbicula fluminea Müller. , 1999, Comparative biochemistry and physiology. Part C, Pharmacology, toxicology & endocrinology.

[4]  A. Bianchini,et al.  Anticholinesterase effect of eserine (physostigmine) in fish and crustacean species , 2001 .

[5]  R. T. Giulio,et al.  The anticholinesterase effect of the cotton defoliant S,S,S-tri-n-butyl phosphorotrithioate (DEF) on channel catfish , 1988 .

[6]  A. Venturino,et al.  Time course of brain cholinesterase inhibition and recovery following acute and subacute azinphosmethyl, parathion and carbaryl exposure in the goldfish (Carassius auratus). , 2004, Ecotoxicology and environmental safety.

[7]  F. Iverson,et al.  Carbamylation and affinity constants of some carbamate inhibitors of acetylcholinesterase and their relation to analogous substrate constants. , 1970, Journal of agricultural and food chemistry.

[8]  A. Main.  Affinity and Phosphorylation Constants for the Inhibition of Esterases by Organophosphates , 1964, Science.

[9]  Fan De-fang,et al.  Correlation between biochemical parameters and susceptibility of freshwater fish to malathion. , 1996, Journal of toxicology and environmental health.

[10]  K. Courtney,et al.  A new and rapid colorimetric determination of acetylcholinesterase activity. , 1961, Biochemical pharmacology.

[11]  D. Livingstone.  The fate of organic xenobiotics in aquatic ecosystems: quantitative and qualitative differences in biotransformation by invertebrates and fish. , 1998, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology.

[12]  H. Kolm,et al.  Spatial Variation of Bacteria in Surface Waters of Paranaguá and Antonina Bays, Paraná, Brazil , 2002 .

[13]  J. Garric,et al.  The measurement of cholinesterase activities as a biomarker in chub (Leuciscus cephalus): the fish length should not be ignored. , 2002, Environmental pollution.

[14]  G. Chuiko,et al.  Acetylcholinesterase and butyrylcholinesterase activities in brain and plasma of freshwater teleosts: cross-species and cross-family differences. , 2003, Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology.

[15]  A. Bianchini,et al.  Some kinetic and toxicological characteristics of thoracic ganglia cholinesterase of Chasmagnathus granulata (Decapoda, Grapsidae). , 1998, Comparative biochemistry and physiology. Part C, Pharmacology, toxicology & endocrinology.

[16]  G. Chuiko,et al.  Adrenaline induced changes of acetylcholinesterase activity in the brain of perch (Perca fluviatilis L.) , 1994 .

[17]  A. Salibián,et al.  Freshwater pollution biomarker: response of brain acetylcholinesterase activity in two fish species. , 2002, Comparative biochemistry and physiology. Toxicology & pharmacology : CBP.

[18]  G. Chuiko,et al.  Feeding behavior and brain acetylcholinesterase activity in bream (Abramis brama L.) as affected by DDVP, an organophosphorus insecticide. , 1992, Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology.

[19]  F. Galgani,et al.  Characterization and assay conditions for Use of AChE activity from several marine species in pollution monitoring , 1990 .

[20]  J. Monserrat,et al.  Kinetic and toxicological characteristics of acetylcholinesterase from the gills of oysters (Crassostrea rhizophorae) and other aquatic species. , 2002, Marine environmental research.

[21]  E. Fabbri,et al.  Characterization of cholinesterase activity in three bivalves inhabiting the North Adriatic sea and their possible use as sentinel organisms for biosurveillance programmes. , 2003, The Science of the total environment.

[22]  V. Isaac.  Synopsis of biological data on the whitemouth croaker Micropogonias furnieri (Desmarest, 1823) , 1988 .

[23]  G. Chuiko,et al.  Seasonal Fluctuations in Brain Acetylcholinesterase Activity and Soluble Protein Content in Roach (Rutilus rutilus L.): A Freshwater Fish from Northwest Russia , 1997 .

[24]  A. Sturm,et al.  Cholinesterases of marine teleost fish: enzymological characterization and potential use in the monitoring of neurotoxic contamination , 1999 .

[25]  D. Kaufer,et al.  Anticholinesterases induce multigenic transcriptional feedback response suppressing cholinergic neurotransmission. , 1999, Chemico-biological interactions.

[26]  K. Sloman,et al.  The effects of environmental pollutants on complex fish behaviour: integrating behavioural and physiological indicators of toxicity. , 2004, Aquatic toxicology.

[27]  G. Chuiko,et al.  Comparative study of acetylcholinesterase and butyrylcholinesterase in brain and serum of several freshwater fish: specific activities and in vitro inhibition by DDVP, an organophosphorus pesticide. , 2000, Comparative biochemistry and physiology. Toxicology & pharmacology : CBP.

[28]  J. Navarro,et al.  Effect of dichlorvos on cholinesterase activity of the European sea bass (Dicentrarchus labrax) , 2003 .

[29]  C. Wang,et al.  The role of non-critical binding proteins in the sensitivity of acetylcholinesterase from different species to diisopropyl fluorophosphate (DFP), in vitro. , 1982, Life sciences.

[30]  Distribution of particulate trace metal in the southern part of the Patos Lagoon estuary , 2000 .