Evidence for incomplete replication of a penguin poxvirus in cells of mammalian origin.

The recent discovery of a novel poxvirus [penguin-pox virus (PPV)] from Jackass penguins offers the potential of a unique candidate vaccine vector for use in mammals. Infectivity studies were therefore undertaken using a number of mammalian cell lines and chick embryo fibroblasts (CEF). It was shown that the simian CV-1 cell line was able to support replication of the PPV DNA, but no infectious progeny virus could be recovered from the infected cells. Electron microscopy was used to establish the extent of virus morphogenesis in CV-1 cells as compared to that in both chorio-allantoic membranes (CAMs) of hens' eggs and CEF cells. It appears that CV-1 cells are able to support partial maturation of PPV, but that morphogenesis does not proceed to the stage of mature infectious particles. Vaccinia virus promoters were successful in achieving transient gene expression in PPV-infected cells.

[1]  M. Skinner,et al.  Fowlpox virus host range restriction: gene expression, DNA replication, and morphogenesis in nonpermissive mammalian cells. , 1993, Virology.

[2]  E. Paoletti,et al.  Induction of cytotoxic T lymphocytes by recombinant canarypox (ALVAC) and attenuated vaccinia (NYVAC) viruses expressing the HIV-1 envelope glycoprotein. , 1993, Virology.

[3]  R. Doms,et al.  Assembly of vaccinia virus: role of the intermediate compartment between the endoplasmic reticulum and the Golgi stacks , 1993, The Journal of cell biology.

[4]  E. Paoletti,et al.  Protection of cats against feline leukemia virus by vaccination with a canarypox virus recombinant, ALVAC-FL , 1993, Journal of virology.

[5]  B. Moss,et al.  Nonreplicating vaccinia vector efficiently expresses recombinant genes. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[6]  E. Paoletti,et al.  Immunisation with canarypox virus expressing rabies glycoprotein , 1992, The Lancet.

[7]  E. Paoletti,et al.  Nonreplicating viral vectors as potential vaccines: recombinant canarypox virus expressing measles virus fusion (F) and hemagglutinin (HA) glycoproteins. , 1992, Virology.

[8]  D. Kow.  Characterization of avipoxviruses for use in recombinant vaccines , 1992 .

[9]  E. Paoletti,et al.  Potential use of non-replicating vectors as recombinant vaccines. , 1992, Vaccine.

[10]  J. Roujeau,et al.  Vaccinia from recombinant virus expressing HIV genes , 1991, The Lancet.

[11]  E. Paoletti,et al.  Efficacy studies on a canarypox-rabies recombinant virus. , 1991, Vaccine.

[12]  G. Nolan,et al.  Use of Escherichiu coli (E. coli) lacZ (β-Galactosidase) as a Reporter Gene. , 1991, Methods in molecular biology.

[13]  S. Goebel,et al.  Vaccinia virus host range genes. , 1990, Virology.

[14]  M. Binns,et al.  Analysis of the fowlpoxvirus gene encoding the 4b core polypeptide and demonstration that it possesses efficient promoter sequences. , 1989, Virology.

[15]  C. Caskey,et al.  Construction of plasmids that express Escherichia coli β-galactosidase in mammalian cells , 1989 .

[16]  E. Paoletti,et al.  Recombinant fowlpox virus inducing protective immunity in non-avian species. , 1988, Vaccine.

[17]  D. Spehner,et al.  A cowpox virus gene required for multiplication in Chinese hamster ovary cells , 1988, Journal of virology.

[18]  T. S. Jones,et al.  Disseminated vaccinia in a military recruit with human immunodeficiency virus (HIV) disease. , 1987, The New England journal of medicine.

[19]  S. Dales,et al.  Biogenesis of vaccinia: relationship of the envelope to virus assembly. , 1976, Virology.

[20]  W. Russell,et al.  A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses , 1975, Nature.

[21]  D. Hughes,et al.  The morphogenesis of pigeonpox virus. , 1972, The Journal of general virology.

[22]  N. Cheville.  Cytopathologic changes in fowlpox (turkey origin) inclusion body formation. , 1966, The American journal of pathology.

[23]  C. Randall,et al.  Electron microscopic observations on the development of fowlpox virus in chorioallantoic membrane , 1964 .

[24]  W. Joklik.  The purification fo four strains of poxvirus. , 1962, Virology.

[25]  F. Fenner,et al.  A short description of the poxvirus group (vaccinia and related viruses). , 1957, Virology.