Transmission electron microscopic observation of cells cultured on multiwalled carbon nanotube-coated sponges.

The cell structure and interface between cultured cells and a multiwalled carbon nanotube (MWCNT)-coated sponge (MWCNT-coated sponge) were observed by transmission electron microscopy (TEM). Moreover, the atomic structure of MWCNTs that entered the cells was also examined by means of high-resolution TEM (HRTEM). MWCNTs were observed in the cytoplasm, and a few MWCNTs were recognized in the cell nuclei. Those MWCNTs maintained their structure there. Subcellular organelles did not appear to be different from those on the collagen sponge despite the cellular uptake of MWCNTs.

[1]  Kang Sun,et al.  Cell-specific cytotoxicity of dextran-stabilized magnetite nanoparticles. , 2010, Colloids and surfaces. B, Biointerfaces.

[2]  Y. Nodasaka,et al.  3D collagen scaffolds coated with multiwalled carbon nanotubes: initial cell attachment to internal surface. , 2010, Journal of biomedical materials research. Part B, Applied biomaterials.

[3]  Bing Yan,et al.  Endosomal leakage and nuclear translocation of multiwalled carbon nanotubes: developing a model for cell uptake. , 2009, Nano letters.

[4]  H. Takita,et al.  Development of a 3D collagen scaffold coated with multiwalled carbon nanotubes. , 2009, Journal of biomedical materials research. Part B, Applied biomaterials.

[5]  Y. Nodasaka,et al.  High-resolution electron microscopy of multi-wall carbon nanotubes in the subcutaneous tissue of rats. , 2008, Journal of electron microscopy.

[6]  V. Barron,et al.  Carbon nanotubes and mesenchymal stem cells: biocompatibility, proliferation and differentiation. , 2008, Nano letters.

[7]  L. J. Terry,et al.  Crossing the Nuclear Envelope: Hierarchical Regulation of Nucleocytoplasmic Transport , 2007, Science.

[8]  M. Prato,et al.  Cellular uptake of functionalized carbon nanotubes is independent of functional group and cell type. , 2007, Nature nanotechnology.

[9]  Hui Hu,et al.  Bone cell proliferation on carbon nanotubes. , 2006, Nano letters.

[10]  Zhuang Liu,et al.  Carbon nanotubes as intracellular transporters for proteins and DNA: an investigation of the uptake mechanism and pathway. , 2006, Angewandte Chemie.

[11]  M. Prato,et al.  Biomedical applications of functionalised carbon nanotubes. , 2005, Chemical communications.

[12]  A. Thie,et al.  Fabrication and Biocompatibility of Carbon Nanotube-Based 3D Networks as Scaffolds for Cell Seeding and Growth , 2004 .

[13]  M. Prato,et al.  Functionalized carbon nanotubes for plasmid DNA gene delivery. , 2004, Angewandte Chemie.

[14]  Francisco del Monte,et al.  Multiwall carbon nanotube scaffolds for tissue engineering purposes. , 2008, Biomaterials.