Effects of Sub-Lethal Concentrations of Diazinon on Total Protein and Transaminase Activities in Clarias gariepinus

Diazinon-induced changes in the total protein and transaminase activities of Clarias gariepinus, a common Niger Delta wetland fish were assessed. Adult fish (mean length 35.24±2.80 cm) were acclimatized to laboratory conditions for 7 days and then exposed to varying sub-lethal concentrations of diazinon (1.0, 2.5, 5.0, 7.5 and 10 .0 mg /L) in semi-static bioassays for 30 d ays. Total protein and the activities of aspa rtate aminotransferase (AST) and alanine aminotransferase (ALT) were determined in plasma, muscle, liver, gills and kidney. The levels of total protein in plasma and muscle were significantly lower (p<0.05) in al l test concentrations in comparison with the control; but no concentration-dependent in depletions were observed. On the other hand, protein concentrations in liver, kidney and gills decreased with increased concentration of diazinon. In addition to significant differences in ALT and AST in liver, kidney, gills and muscle when compared with the control, concentration dependent activities in ALT was observed to in the liver and gills. It is concluded that protein concentration as well as ALT activities in gills and liver of Clarias gariepinus are more useful biomarkers of sub-lethal effects of diazinon than total protein, ALT and AST activities in plasma.

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