Combined far-field, near-field and topographic imaging of nano-engineered polyelectrolyte capsules

Abstract Nano-engineered polyelectrolyte capsules (NPCs) were investigated using a multimodal approach based on Confocal Laser Scanning Microscopy, scattering-type Scanning Near-Field Optical Microscopy and Atomic Force Microscopy. Our experiment demonstrates that correlating optical information collected with far-field and near-field techniques and topographic data collected with scanning probe variants holds significant potential for gaining new insights on the structure of NPCs assembled with layer-by-layer strategies.

[1]  Gleb B. Sukhorukov,et al.  LAYER-BY-LAYER SELF ASSEMBLY OF POLYELECTROLYTES ON COLLOIDAL PARTICLES , 1998 .

[2]  G. Sukhorukov,et al.  Improved and targeted delivery of bioactive molecules to cells with magnetic layer-by-layer assembled microcapsules. , 2015, Nanoscale.

[3]  S. Stanciu,et al.  High-resolution quantitative determination of dielectric function by using scattering scanning near-field optical microscopy , 2015, Scientific Reports.

[4]  Raimo Hartmann,et al.  Multiple internalization pathways of polyelectrolyte multilayer capsules into mammalian cells. , 2013, ACS nano.

[5]  S. Stanciu,et al.  Mapping electron-beam-injected trapped charge with scattering scanning near-field optical microscopy. , 2016, Optics letters.

[6]  H. Möhwald,et al.  Multifunctional cargo systems for biotechnology. , 2007, Trends in biotechnology.

[7]  S. Tofail,et al.  Multilayered Polyelectrolyte Microcapsules: Interaction with the Enzyme Cytochrome C Oxidase , 2014, PloS one.

[8]  Gleb B. Sukhorukov,et al.  Stepwise Polyelectrolyte Assembly on Particle Surfaces: a Novel Approach to Colloid Design , 1998 .

[9]  Thomas Taubner,et al.  Nanoscale-resolved subsurface imaging by scattering-type near-field optical microscopy. , 2005, Optics express.

[10]  A. Gliozzi,et al.  Encapsulated living cells on microstructured surfaces. , 2005, Langmuir : the ACS journal of surfaces and colloids.

[11]  Fritz Keilmann,et al.  Enhanced dielectric contrast in scattering-type scanning near-field optical microscopy , 2000 .

[12]  G. Sukhorukov,et al.  Protein encapsulation via porous CaCO3 microparticles templating. , 2004, Biomacromolecules.