Cratylia mollis lectin nanoelectrode for differential diagnostic of prostate cancer and benign prostatic hyperplasia based on label-free detection.

The research for new biomarkers of cancer has studied the role of fetuin glycoprotein on the metastatic disease diagnosis. Cratylia mollis is a lectin with high finity to fetuin, and used here to differentiate prostate cancer and benign prostatic hyperplasia. A label-free electrochemical nanosensor based on assembled carboxylated carbon nanotubes (COOH-CNTs) and poly-L-lysine (PLL) film was developed and applied to serum samples of prostate cancer positive for Gleason score. The electrode analytical response to fetuin in PBS samples, obtained by square wave voltammetry, exhibited a linear range from 0.5 to 25µgmL(-1), with a high correlation coefficient (r=0.994, p<0.001) and low limit of detection (0.017µgmL(-1)). The lectin nanoelectrode showed a good repeatability (1.24% RSD) and reproducibility (4.24% RSD). A pool of serum samples from prostate cancer patients with known the Gleason score were tested showing a significant statistically correlation. Thus, the lectin nanoelectrode was able to distinguish the degree of staging prostate cancer, providing the diagnostic differentiation of benign and malign hyperplasia. To the best of our knowledge, it is the first biosensor for this application using a lectin.

[1]  Milan Mikula,et al.  Ultrasensitive impedimetric lectin based biosensor for glycoproteins containing sialic acid , 2012, Microchimica Acta.

[2]  Rosa F. Dutra,et al.  Amino-Functionalization of Carbon Nanotubes by Using a Factorial Design: Human Cardiac Troponin T Immunosensing Application , 2014, BioMed research international.

[3]  Shi Wang,et al.  Electrodeposited indigotetrasulfonate film onto glutaraldehyde-cross-linked poly-l-lysine modified glassy carbon electrode for detection of dissolved oxygen , 2011 .

[4]  Allen J. Bard,et al.  Electrochemical Methods: Fundamentals and Applications , 1980 .

[5]  J. Tkáč,et al.  Nanoscale controlled architecture for development of ultrasensitive lectin biosensors applicable in glycomics. , 2014, Analytical methods : advancing methods and applications.

[6]  S. K. Vashist,et al.  Rapid sandwich ELISA-based in vitro diagnostic procedure for the highly-sensitive detection of human fetuin A. , 2015, Biosensors & bioelectronics.

[7]  J. S. Owen,et al.  Immobilized Cratylia mollis lectin as a potential matrix to isolate plasma glycoproteins, including lecithin-cholesterol acyltransferase , 1997 .

[8]  Yadong Xue,et al.  Noncovalent functionalization of carbon nanotubes with lectin for label-free dynamic monitoring of cell-surface glycan expression. , 2011, Analytical biochemistry.

[9]  C. A. Andrade,et al.  Detection of dengue virus serotypes on the surface of gold electrode based on Cratylia mollis lectin affinity , 2011 .

[10]  Zhigang Zhu,et al.  A Critical Review of Glucose Biosensors Based on Carbon Nanomaterials: Carbon Nanotubes and Graphene , 2012, Sensors.

[11]  R. Sidman,et al.  Discovery and horizontal follow-up of an autoantibody signature in human prostate cancer , 2015, Proceedings of the National Academy of Sciences.

[12]  T. H. van der Kwast,et al.  EAU guidelines on prostate cancer. part 1: screening, diagnosis, and local treatment with curative intent-update 2013. , 2014, European urology.

[13]  V. Moyer,et al.  Screening for Prostate Cancer: U.S. Preventive Services Task Force Recommendation Statement , 2012, Annals of Internal Medicine.

[14]  R. F. Dutra,et al.  A carbon nanotube-based electrochemical immunosensor for cardiac troponin T , 2013 .

[15]  Yue Fan,et al.  Core fucosylation and alpha2-3 sialylation in serum N-glycome is significantly increased in prostate cancer comparing to benign prostate hyperplasia. , 2011, Glycobiology.

[16]  M. Roehrl,et al.  Glycoproteomic Analysis of Human Lung Adenocarcinomas Using Glycoarrays and Tandem Mass Spectrometry: Differential Expression and Glycosylation Patterns of Vimentin and Fetuin A Isoforms , 2009, The protein journal.

[17]  T. Fujigaya,et al.  Non-covalent polymer wrapping of carbon nanotubes and the role of wrapped polymers as functional dispersants , 2015, Science and technology of advanced materials.

[18]  A. B. Reese,et al.  Management of retinoblastoma , 1964, Annals of the New York Academy of Sciences.

[19]  Jun Liu,et al.  Glucose oxidase-graphene-chitosan modified electrode for direct electrochemistry and glucose sensing. , 2009, Biosensors & bioelectronics.

[20]  Sergei Svarovsky,et al.  Label-free impedimetric detection of glycan-lectin interactions. , 2007, Analytical chemistry.

[21]  L. Coelho,et al.  Purification of a glucose/mannose specific lectin, isoform 1, from seeds of Cratylia mollis mart. (Camaratu Bean) , 1995, Applied biochemistry and biotechnology.

[22]  O. J. Semmes,et al.  Lectin Capture Strategies Combined with Mass Spectrometry for the Discovery of Serum Glycoprotein Biomarkers* , 2006, Molecular & Cellular Proteomics.

[23]  L. Kubota,et al.  Electrochemical detection of dengue virus NS1 protein with a poly(allylamine)/carbon nanotube layered immunoelectrode , 2015 .

[24]  Xiaoqiang Liu,et al.  Square wave voltammetry versus electrochemical impedance spectroscopy as a rapid detection technique at electrochemical immunosensors. , 2010, Biosensors & bioelectronics.

[25]  N. A. Siddiqui,et al.  DISPERSION AND FUNCTIONALIZATION OF CARBON NANOTUBES FOR POLYMER-BASED NANOCOMPOSITES: A REVIEW , 2010 .

[26]  Maria D. L. Oliveira,et al.  Electrochemical evaluation of lectin-sugar interaction on gold electrode modified with colloidal gold and polyvinyl butyral. , 2008, Colloids and surfaces. B, Biointerfaces.

[27]  Sandeep Kumar Vashist,et al.  Comparison of 1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide Based Strategies to Crosslink Antibodies on Amine-Functionalized Platforms for Immunodiagnostic Applications , 2012, Diagnostics.

[28]  E. Klein,et al.  The use of treatments for erectile dysfunction among survivors of prostate carcinoma , 2002, Cancer.

[29]  Mohammadali Safavieh,et al.  Toward the development of smart and low cost point-of-care biosensors based on screen printed electrodes , 2015, Critical reviews in biotechnology.

[30]  Tao Yang,et al.  A DNA electrochemical sensor with poly-l-lysine/single-walled carbon nanotubes films and its application for the highly sensitive EIS detection of PAT gene fragment and PCR amplification of NOS gene , 2008 .

[31]  Xin Zhang,et al.  Formation of new biosilica-like structures by flow-induced forces , 2012 .

[32]  Alan W Partin,et al.  Prognostic Gleason grade grouping: data based on the modified Gleason scoring system , 2013, BJU international.

[33]  J. Ochieng,et al.  Fetuin-A (α2HS-Glycoprotein) Is a Major Serum Adhesive Protein That Mediates Growth Signaling in Breast Tumor Cells* , 2010, The Journal of Biological Chemistry.

[34]  D. Noh,et al.  Autoantibody to Tumor Antigen, Alpha 2-HS Glycoprotein: A Novel Biomarker of Breast Cancer Screening and Diagnosis , 2008, Cancer Epidemiology Biomarkers & Prevention.

[35]  S. K. Vashist,et al.  A rapid sandwich immunoassay for human fetuin A using agarose-3-aminopropyltriethoxysilane modified microtiter plate. , 2015, Analytica chimica acta.

[36]  A. Jemal,et al.  Cancer statistics, 2014 , 2014, CA: a cancer journal for clinicians.

[37]  S. K. Vashist,et al.  Surface plasmon resonance-based immunoassay for human fetuin A. , 2014, The Analyst.