Biocompatibility of anionic collagen matrix as scaffold for bone healing.

The basic approach to the treatment of bone defects involves the use of scaffolds to favor tissue growth. Although several bioscaffolds have been proposed for this purpose, the search for new and enhanced materials continues in an attempt to address the drawbacks of the present ones. Modifying current materials can be a fast and cheap way to develop new ones. Among them, type I collagen allows its structure to be modified using relatively simple techniques. By means of an alkaline treatment, anionic collagen with enhanced piezoelectric properties can be obtained through hydrolysis of carboxyamides groups of asparagine and glutamine residues from collagen in carboxylic. The process applied to a raw source of collagen, bovine pericardium, provided a sponge-like structure, with heterogeneous pore size, and, moreover, the complete removal of interstitial cells. For the evaluation of the biocompatibility of such matrices, they were implanted in surgically created bone defects in rat tibias. Empty defects served as controls. This experimental model allowed a preliminary evaluation of the osteoconductiveness of the matrices. The histological results presented a low inflammatory response and bone formation within a short period of time, similar to that of controls. The low cost of production associated to the biocompatibility and osteoconductivity performance make the anionic collagen matrices promising alternatives for bone defects treatment.

[1]  E. Beachey,et al.  Collagen--platelet interaction. , 1979, International review of connective tissue research.

[2]  M. Citardi,et al.  Nonvascularized autogenous bone grafts for craniofacial skeletal augmentation and replacement. , 1994, Otolaryngologic clinics of North America.

[3]  K. Hong,et al.  Osteoconduction at porous hydroxyapatite with various pore configurations. , 2000, Biomaterials.

[4]  M. Rossi,et al.  Lipid extraction attenuates the calcific degeneration of bovine pericardium used in cardiac valve bioprostheses. , 1990, Journal of experimental pathology.

[5]  P. K. Sehgal,et al.  Cellular proliferation on desamidated collagen matrices. , 1999, Comparative biochemistry and physiology. Part C, Pharmacology, toxicology & endocrinology.

[6]  B. Strates,et al.  Chemically modified collagen: a natural biomaterial for tissue replacement. , 1987, Journal of biomedical materials research.

[7]  G. Góissis,et al.  Hidrólise seletiva de carboxiamidas de resíduos de asparagina e glutamina em colágeno: preparação e caracterização de matrizes aniônicas para uso como biomateriais , 1998 .

[8]  J. Pachence,et al.  Collagen-based devices for soft tissue repair. , 1996, Journal of biomedical materials research.

[9]  T. Chiang Collagen-platelet interaction: platelet non-integrin receptors. , 1999, Histology and Histopathology.

[10]  J. H. Lee,et al.  Interaction of cells on chargeable functional group gradient surfaces. , 1997, Biomaterials.

[11]  S. Watson Collagen Receptor Signaling in Platelets and Megakaryocytes , 1999, Thrombosis and Haemostasis.

[12]  John P. Bilezikian,et al.  Principles of Bone Biology , 1996 .

[13]  C. Rappaport,et al.  Studies on properties of surfaces required for growth of mammalian cells in synthetic medium. I. The HeLa cell. , 1960, Experimental cell research.

[14]  M. Swiontkowski,et al.  Bone-graft substitutes , 1999, The Lancet.

[15]  J. Adrian,et al.  Collagen gel in osseous defects. A preliminary study. , 1976, Oral surgery, oral medicine, and oral pathology.

[16]  Olivier Gauthier,et al.  Macroporous biphasic calcium phosphate ceramics , 1997 .

[17]  E. Qwarnstrom,et al.  The role of proteoglycans in cell adhesion, migration and proliferation. , 1992 .

[18]  R. F. Morgan,et al.  Repair of a Rodent Nasal Critical‐Size Osseous Defect With Osteoblast Augmented Collagen Gel , 1999, The Laryngoscope.

[19]  F. Kamer,et al.  Clinical use of injectable collagen. A three-year retrospective review. , 1984, Archives of otolaryngology.

[20]  A. Luster,et al.  Chemokines--chemotactic cytokines that mediate inflammation. , 1998, The New England journal of medicine.

[21]  S. T. Smith,et al.  Reaction to Injectable Collagen: Results in Animal Models and Clinical Use , 1987, Plastic and reconstructive surgery.

[22]  C. Friedman,et al.  Synthetic bone graft substitutes. , 1994, Otolaryngologic clinics of North America.

[23]  J. Stehlík,et al.  Morphological features of bone healing under the effect of collagen-graft-glycosaminoglycan copolymer supplemented with the tripeptide Gly-His-Lys. , 1996, Biomaterials.

[24]  C. Rappaport Studies on properties of surfaces required for growth of mammalian cells in synthetic medium. II. The monkey kidney cell. , 1960, Experimental cell research.

[25]  A Oosterhof,et al.  Preparation and characterization of porous crosslinked collagenous matrices containing bioavailable chondroitin sulphate. , 1999, Biomaterials.

[26]  D. Das-gupta,et al.  Dielectric and pyroelectric characterization of anionic and native collagen , 1996 .

[27]  D. Das-gupta,et al.  Anionic collagen: polymer composites with improved dielectric and rheological properties. , 1998, Artificial organs.

[28]  M. DeLacure Physiology of bone healing and bone grafts. , 1994, Otolaryngologic clinics of North America.

[29]  R. Mitchell An evaluation of bone healing in cavities in the jaws implanted with a collagen matrix. , 1992, British Journal of Oral and Maxillofacial Surgery.

[30]  M. Rossi,et al.  Calcific degeneration of pericardial valvular xenografts implanted subcutaneously in rats. , 1986, International journal of cardiology.

[31]  J. Davies,et al.  The migration of osteoblasts over substrata of discrete surface charge. , 1986, Biomaterials.

[32]  R L Jackson,et al.  Glycosaminoglycans: molecular properties, protein interactions, and role in physiological processes. , 1991, Physiological reviews.

[33]  G. Daculsi,et al.  Macroporous biphasic calcium phosphate ceramics: influence of macropore diameter and macroporosity percentage on bone ingrowth. , 1998, Biomaterials.

[34]  P. K. Sehgal,et al.  Studies on the desamidation of bovine collagen. , 1997, Journal of biomedical materials research.