Silver development in microscopy and bioanalysis: past and present

With the experience accumulated from more than a century of silver applications in biology and medicine, physical development has become a powerful bioanalytical tool for marker amplification in blotting procedures, in situ hybridization, immunocytochemistry, histochemistry, and cytochemistry. Early, empirical techniques of silver impregnation followed by development in a reducing solution (chemical developer), or a solution which contained both silver reducers and silver salts (physical developer) were often capricious and suffered from unwanted silver precipitation caused by light and self‐nucleation. To accommodate the modern demand for accurate physical development, various strategies have been devised to counter these problems. One approach has been to introduce organic colloids into the developer to keep the silver ions and reducer molecules apart, whilst excluding light by using a dark‐room or by covering the solution. Albumen, gelatin, and complex polysaccharides have all been tested, but gum arabic is preferred. In addition, further control can be achieved by slowing down the rate of development with low pH and by changing from silver nitrate to silver lactate, which dissociates more slowly. Effective colloid protection in a physical developer is also provided by the inclusion of tungsten salts which can delay light‐catalysed silver reduction and keep the developer clear for many minutes. The same result has been achieved by complexing the silver salt in the physical developer with very large organic molecules, restricting ionization. ‘Light insensitive’ commercial designer products have resulted. Probably no single formulation can satisfy all conditions of use, but with increased understanding of the mechanisms of physical developers a more flexible, user‐friendly approach is anticipated. Copyright © 1998 John Wiley & Sons, Ltd.

[1]  A. Graybiel,et al.  A differentiated silver intensification procedure for the peroxidase-diaminobenzidine reaction. , 1996, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[2]  M. Stoltenberg,et al.  How to detect gold, silver and mercury in human brain and other tissues by autometallographic silver amplification , 1994, Neuropathology and applied neurobiology.

[3]  G. Danscher,et al.  Autometallographic Localization of Synaptic Vesicular Zinc and Lysosomal Gold, Silver and Mercury , 1994 .

[4]  P. Rivas-Manzano,et al.  Microwaves applied to silver impregnations with ammoniacal silver carbonate. , 1994, Biotechnic & histochemistry : official publication of the Biological Stain Commission.

[5]  G. Buzsáki,et al.  Four modified silver methods for thick sections of formaldehyde-fixed mammalian central nervous tissue: ‘dark’ neurons, perikarya of all neurons, microglial cells and capillaries , 1993, Journal of Neuroscience Methods.

[6]  G. Danscher,et al.  Autometallographic Silver Amplification of Colloidal Gold , 1993 .

[7]  P. Goldman-Rakic,et al.  Silver-enhanced diaminobenzidine-sulfide (SEDS): a technique for high-resolution immunoelectron microscopy demonstrated with monoamine immunoreactivity in monkey cerebral cortex and caudate. , 1993, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[8]  R. Kittelberger,et al.  Sensitive silver-staining detection of bacterial lipopolysaccharides in polyacrylamide gels. , 1993, Journal of biochemical and biophysical methods.

[9]  F. Gallyas,et al.  Skimmed‐milk blocking improves silver post‐intensification of peroxidase‐diaminobenzidine staining on nitrocellulose membrane in immunoblotting , 1993, Electrophoresis.

[10]  G. Buzsáki,et al.  Delayed degeneration of the optic tract and neurons in the superior colliculus after forebrain ischemia , 1992, Neuroscience Letters.

[11]  G. Danscher,et al.  Autometallographic detection of gold in dorsal root ganglia of rats treated with sodium aurothiomalate. , 1992, Experimental and molecular pathology.

[12]  C. Meijer,et al.  Application and comparison of silver intensification methods for the diaminobenzidine and diaminobenzidine-nickel endproduct of the peroxidation reaction in immunohistochemistry and in situ hybridization. , 1992, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[13]  G. Danscher,et al.  Retrograde tracing of zinc-containing neurons by selenide ions: a survey of seven selenium compounds. , 1992, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[14]  T. Rabilloud A comparison between low background silver diammine and silver nitrate protein stains , 1992, Electrophoresis.

[15]  G. Danscher,et al.  Mercury in the dorsal root ganglia of rats treated with inorganic or organic mercury. , 1991, Environmental research.

[16]  G. Danscher,et al.  Applications of autometallography to heavy metal toxicology. , 1991, Pharmacology & toxicology.

[17]  G. Danscher,et al.  Localization of mercury in CNS of the rat. IV. The effect of selenium on orally administered organic and inorganic mercury. , 1991, Toxicology and applied pharmacology.

[18]  B. Humbel,et al.  Suitability of different silver enhancement methods applied to 1 nm colloidal gold particles: an immunoelectron microscopic study. , 1991, Journal of electron microscopy technique.

[19]  J. Tolivia,et al.  A new rapid silver impregnation for neuronal bodies on methacrylate sections , 1991, Journal of Neuroscience Methods.

[20]  J. Wolff,et al.  An argyrophil-III method for the selective demonstration of mitochondria in aldehyde-fixed rat brain , 1990, Journal of Neuroscience Methods.

[21]  F. Gallyas,et al.  Trauma‐induced golgi‐like staining of neurons: A new approach to neuronal organization and response to injury , 1990, The Journal of comparative neurology.

[22]  J. Rungby,et al.  Traces of mercury in organs from primates with amalgam fillings. , 1990, Experimental and molecular pathology.

[23]  M. Lyon,et al.  A general method for the detection and mapping of submicrogram quantities of glycosaminoglycan oligosaccharides on polyacrylamide gels by sequential staining with azure A and ammoniacal silver. , 1990, Analytical biochemistry.

[24]  R. Iida,et al.  Intensification of peroxidase‐diaminobenzidine staining using gold‐sulfide‐silver: A rapid and highly sensitive method for visualization in immunoblotting , 1990, Electrophoresis.

[25]  G. Danscher,et al.  Zinc-containing neurons in hippocampus and related CNS structures. , 1990, Progress in brain research.

[26]  F. Gallyas,et al.  A highly sensitive one-step method for silver intensification of the nickel-diaminobenzidine endproduct of peroxidase reaction. , 1989, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[27]  G. Danscher,et al.  Silver enhancement of tissue mercury: demonstration of mercury in autometallographic silver grains from rat kidneys. , 1989, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[28]  H. Heinsen,et al.  Laminar neuropathology in Alzheimer's disease by a modified Gallyas impregnation , 1989, Psychiatry Research.

[29]  L. Morikawa,et al.  Periodic Acid-Ammoniacal Silver (PAAS) Method for the Demonstration of Glomerular Basement Membranes Using the Microwave Oven , 1989 .

[30]  G. Hacker 10 – Silver-Enhanced Colloidal Gold for Light Microscopy , 1989 .

[31]  L. Scopsi 9 – Silver-Enhanced Colloidal Gold Method , 1989 .

[32]  G. Danscher,et al.  Autometallographic demonstration of gold uptake in cultured synovial fluid cells from patients with rheumatoid arthritis. , 1989, Scandinavian journal of rheumatology.

[33]  F. Gallyas,et al.  Copper-H2O2 oxidation strikingly improves silver intensification of the nickel-diaminobenzidine (Ni-DAB) end-product of the peroxidase reaction. , 1988, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[34]  J. Reboud,et al.  Selective silver-staining methods for RNA and proteins in the same polyacrylamide gels. , 1988, Analytical biochemistry.

[35]  N. Schwartz,et al.  An improved method of sequential alcian blue and ammoniacal silver staining of chondroitin sulfate proteoglycan in polyacrylamide gels. , 1987, Analytical biochemistry.

[36]  J. Tolivia,et al.  A new rapid silver impregnation for neuronal bodies , 1987, Journal of Neuroscience Methods.

[37]  D. Przepiorka,et al.  A single-step silver enhancement method permitting rapid diagnosis of cytomegalovirus infection in formalin-fixed, paraffin-embedded tissue sections by in situ hybridization and immunoperoxidase detection. , 1986, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[38]  J. Wolff,et al.  Metal-catalyzed oxidation renders silver intensification selective. Applications for the histochemistry of diaminobenzidine and neurofibrillary changes. , 1986, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[39]  M. Moeremans,et al.  Sequential immunostaining (gold/silver) and complete protein staining (AuroDye) on Western blots. , 1986, Journal of immunological methods.

[40]  E. Schenk,et al.  Dilute Ammoniacal Silver as a Substitute for Methenamine Silver to Demonstrate Pneumocystis carinii and Fungi , 1986 .

[41]  G. Danscher,et al.  Entorhinal and prepiriform cortices of the European hedgehog. A hisstochemical and densitometric study based on a comparison between Timm's sulphide silver method and the selenium method , 1985, Brain Research.

[42]  G. Danscher,et al.  Ultrastructural autometallography: a method for silver amplification of catalytic metals. , 1985, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[43]  G. Danscher,et al.  Intravesicular localization of zinc in rat telencephalic boutons. A histochemical study , 1985, Brain Research.

[44]  G. Danscher,et al.  Silver amplification of mercury sulfide and selenide: a histochemical method for light and electron microscopic localization of mercury in tissue. , 1985, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[45]  M. Moeremans,et al.  Sensitive visualization of antigen-antibody reactions in dot and blot immune overlay assays with immunogold and immunogold/silver staining. , 1984, Journal of immunological methods.

[46]  J. Roth,et al.  "Golden blot"--detection of polyclonal and monoclonal antibodies bound to antigens on nitrocellulose by protein A-gold complexes. , 1984, Analytical biochemistry.

[47]  G. Danscher Autometallography , 2004, Histochemistry.

[48]  G. Danscher,et al.  Light microscopic visualization of colloidal gold on resin-embedded tissue. , 1983, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[49]  B. Jasani,et al.  Metal compound intensification of the electron-density of diaminobenzidine. , 1983, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[50]  B. Jasani,et al.  THE VISUALISATION OF TRACE AMOUNTS OF DIAMINOBENZIDINE (DAB) POLYMER BY A NOVEL GOLD‐SULPHIDE‐SILVER METHOD , 1983 .

[51]  C C Bird,et al.  Immunogold-silver staining: new method of immunostaining with enhanced sensitivity. , 1983, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[52]  I. Lauder,et al.  Surface membrane staining of immunoglobulins in paraffin sections of non-Hodgkin's lymphomas using immunogold-silver staining technique. , 1983, Journal of clinical pathology.

[53]  F. Gallyas,et al.  Improvement of the electron microscopic detection of peroxidase activity by means of the silver intensification of the diaminobenzidine reaction in the rat nervous system , 1982, Neuroscience Letters.

[54]  F. Gallyas,et al.  High-grade intensification of the end-product of the diaminobenzidine reaction for peroxidase histochemistry. , 1982, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[55]  F. Gallyas Suppression of the argyrophil III reaction by mercapto compounds (a prerequisite for the intensification of certain histochemical reactions by physical developers). , 1982, Acta histochemica.

[56]  J. Adams Heavy metal intensification of DAB-based HRP reaction product. , 1981, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[57]  F. Gallyas Determination of the development time for the characterization of the nucleus formation in the argyrophil stainings. , 1980, Acta histochemica.

[58]  J. Wolff,et al.  Experimental studies of mechanisms involved in methods demonstrating axonal and terminal degeneration. , 1980, Stain technology.

[59]  J. Wolff,et al.  A reliable method for demonstrating axonal degeneration shortly after axotomy. , 1980, Stain technology.

[60]  F. Gallyas Chemical nature of the first products (nuclei) of the argyrophil staining. , 1980, Acta histochemica.

[61]  J. Wolff,et al.  A reliable and sensitive method to localize terminal degeneration and lysosomes in the central nervous system. , 1980, Stain technology.

[62]  D. E. Yorde,et al.  A quantitative immunoperoxidase procedure employing energy dispersive x-ray analysis. , 1979, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[63]  F. Gallyas Light insensitive physical developers. , 1979, Stain technology.

[64]  Smejda Haug Fm Heavy metals in the brain. A light microscope study of the rat with Timm's sulphide silver method. Methodological considerations and cytological and regional staining patterns. , 1973 .

[65]  A. Brun,et al.  THE JOURNAL OF HISTOCHEMISTRY AND CYTOCHEMISTRY , 2005 .

[66]  F. Gallyas Silver staining of collagen and reticulin fibres and cerebral capillaries by means of physical development , 1970, Journal of microscopy.

[67]  Jacob S. Hanker,et al.  NONDROPLET ULTRASTRUCTURAL DEMONSTRATION OF CYTOCHROME OXIDASE ACTIVITY WITH A POLYMERIZING OSMIOPHILIC REAGENT, DIAMINOBENZIDINE (DAB) , 1968, The Journal of cell biology.

[68]  A. Brun,et al.  Histochemical demonstration of heavy metals with the sulfide-silver method. A methodological study. , 1968, Acta histochemica.

[69]  S. Falkmer,et al.  Trials to modify the sulfide-silver method for ultrastructural tissue localization of heavy metals. , 1967, Acta histochemica.

[70]  U. Brunk,et al.  The oxidation problem in the sulphide-silver method for histochemical demonstration of metals. , 1967, Acta histochemica.

[71]  B. Hellman,et al.  Reactions of the two types of A cells in the islets of Langerhans after administration of glucagon. , 1962, Acta endocrinologica.

[72]  B. Hellman,et al.  Some aspects of silver impregnation of the islets of Langerhans in the rat. , 1960, Acta endocrinologica.

[73]  H. S. Garven,et al.  The silver diammine ion staining of peripheral nerve elements and the interpretation of the results: with a modification of the Bielschowsky-Gros method for frozen sections. , 1952, Quarterly journal of experimental physiology and cognate medical sciences.

[74]  D. Bodian A new method for staining nerve fibers and nerve endings in mounted paraffin sections , 1936 .

[75]  H. A. Davenport,et al.  The Bielschowsky Staining Technic a Study of the Factors Influencing Its Specificity for Nerve FIBERS , 1933 .

[76]  W. M. Rogers,et al.  New silver methods for paraffin sections , 1931 .

[77]  H. A. Davenport STAINING NERVE FIBERS IN MOUNTED SECTIONS WITH ALCOHOLIC SILVER NITRATE SOLUTION , 1930 .

[78]  H. A. Davenport Silver impregnation of nerve fibers in celloidin sections , 1929 .

[79]  R. Liesegang Die Kolloidchemie der histologischen Silberfärbungen , 1911 .