Experimental methods of evaluating biofouling of marine benthic diatoms on non-toxic antifouling coating

As a studied marine fouling microorganism,the benthic diatom were cultured in the laboratory.The surface occupied by benthic diatom on non-toxic antifouling coat with the low surface energy,were investigated by a microscope and image processing technology in this paper.In addition,chlorophyll values in benthic diatom were measured.The results showed that the experimental methods proposed in this article are effective for evaluating the settlement of marine biofouling.The measured chlorophyll value in benthic diatom is related linearly to the fouling surface.