Evaluation of Neisseria gonorrhoeae Multiple-Locus Variable-Number Tandem-Repeat Analysis, N. gonorrhoeae Multiantigen Sequence Typing, and Full-Length porB Gene Sequence Analysis for Molecular Epidemiological Typing

ABSTRACT The performance characteristics of Neisseria gonorrhoeae multilocus variable-number tandem-repeat analysis were evaluated, by comparison with N. gonorrhoeae multiantigen sequence typing and full-length porB sequence typing. Assessment of the relatedness of intra- and interpatient isolates showed that all three genotyping techniques display a high resolution and typeability.

[1]  M. Unemo,et al.  Review and International Recommendation of Methods for Typing Neisseria gonorrhoeae Isolates and Their Implications for Improved Knowledge of Gonococcal Epidemiology, Treatment, and Biology , 2011, Clinical Microbiology Reviews.

[2]  M. Unemo,et al.  Ceftriaxone treatment failure of pharyngeal gonorrhoea verified by international recommendations, Sweden, July 2010. , 2011, Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin.

[3]  M. Yen,et al.  Draft Genome Sequence of a Dominant, Multidrug-Resistant Neisseria gonorrhoeae Strain, TCDC-NG08107, from a Sexual Group at High Risk of Acquiring Human Immunodeficiency Virus Infection and Syphilis , 2011, Journal of bacteriology.

[4]  M. Unemo,et al.  Two cases of verified clinical failures using internationally recommended first-line cefixime for gonorrhoea treatment, Norway, 2010. , 2010, Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin.

[5]  L. Schouls,et al.  Multiple-Locus Variable-Number Tandem Repeat Analysis of Neisseria gonorrhoeae , 2010, Journal of Clinical Microbiology.

[6]  M. Unemo,et al.  Emergence, spread and characteristics of Neisseria gonorrhoeae isolates with in vitro decreased susceptibility and resistance to extended-spectrum cephalosporins in Sweden , 2010, Sexually Transmitted Infections.

[7]  E. Falk,et al.  Restriction endonuclease fingerprinting of chromosomal DNA of Neisseria gonorrhoeae. , 2009, Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology.

[8]  M. Unemo,et al.  Phenotypic and genetic characterization of the 2008 WHO Neisseria gonorrhoeae reference strain panel intended for global quality assurance and quality control of gonococcal antimicrobial resistance surveillance for public health purposes. , 2009, The Journal of antimicrobial chemotherapy.

[9]  A. Jolly,et al.  Comparison of Neisseria gonorrhoeae Multiantigen Sequence Typing and porB Sequence Analysis for Identification of Clusters of N. gonorrhoeae Isolates , 2008, Journal of Clinical Microbiology.

[10]  M. Unemo,et al.  The Neisseria gonorrhoeae population in Sweden during 2005—phenotypes, genotypes and antibiotic resistance , 2008, APMIS : acta pathologica, microbiologica, et immunologica Scandinavica.

[11]  K. Jolley,et al.  Species status of Neisseria gonorrhoeae: evolutionary and epidemiological inferences from multilocus sequence typing , 2007, BMC Biology.

[12]  M. Unemo,et al.  Neisseria gonorrhoeae population in Arkhangelsk, Russia: phenotypic and genotypic heterogeneity. , 2007, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.

[13]  A. K. van der Bij,et al.  Molecular Epidemiology of Neisseria gonorrhoeae in Amsterdam, The Netherlands, Shows Distinct Heterosexual and Homosexual Networks , 2006, Journal of Clinical Microbiology.

[14]  L. Schouls,et al.  Multiple-Locus Variable-Number Tandem Repeat Analysis of Neisseria meningitidis Yields Groupings Similar to Those Obtained by Multilocus Sequence Typing , 2006, Journal of Clinical Microbiology.

[15]  K. Fenton,et al.  Increasing rates of sexually transmitted diseases in homosexual men in Western europe and the United States: why? , 2005, Infectious disease clinics of North America.

[16]  M. Unemo,et al.  Molecular Typing of Neisseria gonorrhoeae Isolates by Pyrosequencing of Highly Polymorphic Segments of the porB Gene , 2004, Journal of Clinical Microbiology.

[17]  Kevin A Fenton,et al.  Rapid sequence-based identification of gonococcal transmission clusters in a large metropolitan area. , 2004, The Journal of infectious diseases.

[18]  M. Unemo,et al.  Comparison of Serologic and Genetic porB-Based Typing of Neisseria gonorrhoeae: Consequences for Future Characterization , 2003, Journal of Clinical Microbiology.

[19]  M. Unemo,et al.  Molecular Epidemiology of Neisseria gonorrhoeae: Sequence Analysis of the porB Gene Confirms Presence of Two Circulating Strains , 2002, Journal of Clinical Microbiology.

[20]  A. J. Schultz,et al.  Use of the Neisserial Lipoprotein (Lip) for Subtyping Neisseria gonorrhoeae , 2000, Journal of Clinical Microbiology.

[21]  R. Coutinho,et al.  [Substantial increase in gonorrhea and syphilis among clients of Amsterdam Sexually Transmitted Diseases Clinic]. , 2000, Nederlands tijdschrift voor geneeskunde.

[22]  C. Ison Genotyping of Neisseria gonorrhoeae. , 1998, Current opinion in infectious diseases.

[23]  B. Spratt,et al.  Opa‐typing: a high‐resolution tool for studying the epidemiology of gonorrhoea , 1995, Molecular microbiology.

[24]  J. Dillon,et al.  Utility of ribotyping, restriction endonuclease analysis and pulsed-field gel electrophoresis to discriminate between isolates of Neisseria gonorrhoeae of serovar IA-2 which require arginine, hypoxanthine or uracil for growth. , 1995, Journal of medical microbiology.

[25]  M. Rahman,et al.  Discriminatory power of typing schemes based on Simpson's index of diversity for Neisseria gonorrhoeae , 1993, Journal of clinical microbiology.

[26]  Q. Lau,et al.  Subtyping of Neisseria gonorrhoeae auxotype-serovar groups by pulsed-field gel electrophoresis. , 1993, Journal of medical microbiology.

[27]  P. François,et al.  High-throughput typing of Staphylococcus aureus by amplified fragment length polymorphism (AFLP) or multi-locus variable number of tandem repeat analysis (MLVA) reveals consistent strain relatedness , 2008, European Journal of Clinical Microbiology & Infectious Diseases.

[28]  A. van Belkum Tracing isolates of bacterial species by multilocus variable number of tandem repeat analysis (MLVA). , 2007, FEMS immunology and medical microbiology.

[29]  W. Bossert,et al.  The Measurement of Diversity , 2001 .