Fabrication and characterization of microfibrillated cellulose and collagen composite films

Microfibrillated cellulose (MFC)-collagen composite films were prepared with a dispersion of acid swollen collagen fibers and carboxylated MFC at different ratios in an alkaline homogenous system. The surface topographic results obtained from SEM analyses indicated that the MFC entangled uniformly with collagen in the film and formed a closely interwoven network to reinforce the film structure. However, the MFC addition decreased the smoothness and light transparency of the films due to the aggregation of MFC. Compared to the film prepared with pure collagen, the hybrid composite film showed a higher strength and Young’s modulus but lower elongation. The swelling of the composite film in water increased with the increase of the MFC ratio in the film matrix. DSC and TG analyses demonstrated that adding MFC to collagen benefited the thermal stability of the films, due to the conformational and crystal changes in the MFC/collagen structure indicated by the FT-IR and XRD results. The MFC/collagen composite film can potentially be used as an edible material in the food and packaging industry, in particular for meat products.

[1]  Y. Ni,et al.  Physical crosslinkings of edible collagen casing. , 2015, International journal of biological macromolecules.

[2]  R. Villalobos-Carvajal,et al.  Development of a novel sausage casing , 2015 .

[3]  D. Panaitescu,et al.  Morphology and thermal stability of bacterial cellulose/collagen composites , 2014 .

[4]  Wei Xue,et al.  Preparation and properties of cellulose nanocrystals reinforced collagen composite films. , 2014, Journal of biomedical materials research. Part A.

[5]  A. Gnanamani,et al.  Exploring the dual role of α,ω-di-carboxylic acids in the preparation of collagen based biomaterial , 2013, Journal of Porous Materials.

[6]  S. Russell,et al.  Photo-active collagen systems with controlled triple helix architecture. , 2013, Journal of materials chemistry. B.

[7]  J. Loo,et al.  Collagen–cellulose composite thin films that mimic soft-tissue and allow stem-cell orientation , 2013, Journal of Materials Science: Materials in Medicine.

[8]  F. Cotana,et al.  Production of nanocrystalline cellulose from lignocellulosic biomass: technology and applications. , 2013, Carbohydrate polymers.

[9]  Pan Liu,et al.  Fabrication, properties and bioapplications of cellulose/collagen hydrolysate composite films. , 2013, Carbohydrate polymers.

[10]  P. K. Sehgal,et al.  Synthesis and Characterization of Hybrid Biodegradable Films From Bovine Hide Collagen and Cellulose Derivatives for Biomedical Applications , 2013 .

[11]  S. Ribeiro,et al.  Bacterial cellulose-collagen nanocomposite for bone tissue engineering , 2012 .

[12]  A. Gnanamani,et al.  Preparation and characterization of malonic acid cross-linked chitosan and collagen 3D scaffolds: an approach on non-covalent interactions , 2012, Journal of Materials Science: Materials in Medicine.

[13]  K. Oksman,et al.  Crosslinked fibrous composites based on cellulose nanofibers and collagen with in situ pH induced fibrillation , 2012, Cellulose.

[14]  M. López-Caballero,et al.  Functional and bioactive properties of collagen and gelatin from alternative sources: A review , 2011 .

[15]  Ashlie Martini,et al.  Cellulose nanomaterials review: structure, properties and nanocomposites. , 2011, Chemical Society reviews.

[16]  A. Gnanamani,et al.  Preparation and characterization of a thermostable and biodegradable biopolymers using natural cross-linker. , 2011, International journal of biological macromolecules.

[17]  David Plackett,et al.  Microfibrillated cellulose and new nanocomposite materials: a review , 2010 .

[18]  Moncy V. Jose,et al.  Fabrication and characterization of aligned nanofibrous PLGA/Collagen blends as bone tissue scaffolds , 2009 .

[19]  J. H. Rohling,et al.  Collagen films from swim bladders: preparation method and properties. , 2008, Colloids and surfaces. B, Biointerfaces.

[20]  Luke P. Lee,et al.  Microfluidic alignment of collagen fibers for in vitro cell culture , 2006, Biomedical microdevices.

[21]  B. Ninham,et al.  Why forces between proteins follow different Hofmeister series for pH above and below pI. , 2005, Biophysical chemistry.

[22]  M. Vignon,et al.  Topochemistry of carboxylated cellulose nanocrystals resulting from TEMPO-mediated oxidation , 2005 .

[23]  Alain Dufresne,et al.  Review of recent research into cellulosic whiskers, their properties and their application in nanocomposite field. , 2005, Biomacromolecules.

[24]  H. Fink,et al.  Structure formation of regenerated cellulose materials from NMMO-solutions , 2001 .

[25]  Yugyung Lee,et al.  Biomedical applications of collagen. , 2001, International journal of pharmaceutics.

[26]  R B Rucker,et al.  Enzymatic and nonenzymatic cross‐linking of collagen and elastin , 1992, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[27]  S. Kalia,et al.  Nanofibrillated cellulose: surface modification and potential applications , 2013, Colloid and Polymer Science.

[28]  S. Ricard-Blum.  The collagen family. , 2011, Cold Spring Harbor perspectives in biology.

[29]  D. Achet,et al.  Determination of the renaturation level in gelatin films , 1995 .