ASSESSMENT OF GENETIC DIVERSITY OF SOME COWPEAS (VIGNA UNGUICULATA L.) CULTIVARS GROWN IN EGYPT BASED ON START CODON-TARGETED (SCOT) MARKERS

The different techniques of molecular markers have proved to play a considerable role in assessed the genetic diversity between and within different species. The genetic diversity among the seven cowpea cultivars was characterized using the twelve SCoT markers. The total number of amplicons was 169 with an average of 16.9. Among 169 amplicons, 121 were polymorphic with a level of polymorphism of 71.60%. All primers (12) were successful to identify each of the seven cowpea genotypes by unique positive and/or negative markers. Tiba cultivar showed the highest (15) number of unique markers. The presence of SCoT unique markers among the cowpea cultivars indicates the utility of the approach for fingerprinting purposes. Moreover, the dendrograms constructed from UPGMA divided the seven genotypes into two clusters. The first cluster comprises the cultivar Tiba, Dokki331 and Kaha-1. While the second cluster divided into two subclster the Kareemy7 (subcluster) was separated from other remaining 3 cultivars. The second subcluster contained Balady, Sudan and Kafr-ElShikh, two cultivars that clustered together (Sudany and Balady).

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