Real-time monitoring of geosmin and 2-methylisoborneol, representative odor compounds in water pollution using bioelectronic nose with human-like performance.

A bioelectronic nose for the real-time assessment of water quality was constructed with human olfactory receptor (hOR) and single-walled carbon nanotube field-effect transistor (swCNT-FET). Geosmin (GSM) and 2-methylisoborneol (MIB), mainly produced by bacteria, are representative odor compounds and also indicators of contamination in the water supply system. For the screening of hORs which respond to these compounds, we performed CRE-luciferase assays of the two odorants in heterologous cell system. Human OR51S1 for GSM and OR3A4 for MIB were selected, and nanovesicles expressing the hORs on surface were produced from HEK-293 cell. Carbon nanotube field-effect transistor was functionalized with the nanovesicles. The bioelectronic nose was able to selectively detect GSM and MIB at concentrations as low as a 10 ng L(-1). Furthermore, detection of these compounds from the real samples such as tap water, bottled water and river water was available without any pretreatment processes.

[1]  B. Gao,et al.  Simultaneous analysis of five taste and odor compounds in surface water using solid-phase extraction and gas chromatography-mass spectrometry , 2012, Frontiers of Environmental Science & Engineering.

[2]  Paul V. Zimba,et al.  Rapid analysis of geosmin and 2-methylisoborneol in water using solid phase micro extraction procedures , 1998 .

[3]  K. Freeman.  HARMFUL ALGAL BLOOMS: Musty Warnings of Toxicity , 2010, Environmental health perspectives.

[4]  S. Firestein,et al.  How the olfactory bulb got its glomeruli: a just so story? , 2009, Nature Reviews Neuroscience.

[5]  Jong Hyun Lim,et al.  Ion-channel-coupled receptor-based platform for a real-time measurement of G-protein-coupled receptor activities. , 2015, ACS nano.

[6]  Tai Hyun Park,et al.  Recent advances in the development of bioelectronic nose , 2010 .

[7]  Tai Hyun Park,et al.  Ultrasensitive flexible graphene based field-effect transistor (FET)-type bioelectronic nose. , 2012, Nano letters.

[8]  Horst Vogel,et al.  Investigating cellular signaling reactions in single attoliter vesicles. , 2005, Journal of the American Chemical Society.

[9]  J. Provasi,et al.  Odor sensitivity to geosmin enantiomers , 1992 .

[10]  Hanyi Zhuang,et al.  Synergism of Accessory Factors in Functional Expression of Mammalian Odorant Receptors* , 2007, Journal of Biological Chemistry.

[11]  Hanyi Zhuang,et al.  Evaluating cell-surface expression and measuring activation of mammalian odorant receptors in heterologous cells , 2008, Nature Protocols.

[12]  Tai Hyun Park,et al.  A peptide receptor-based bioelectronic nose for the real-time determination of seafood quality. , 2013, Biosensors & bioelectronics.

[13]  Oh Seok Kwon,et al.  Polypyrrole nanotubes conjugated with human olfactory receptors: high-performance transducers for FET-type bioelectronic noses. , 2009, Angewandte Chemie.

[14]  Tai Hyun Park,et al.  Mimicking the human smell sensing mechanism with an artificial nose platform. , 2012, Biomaterials.

[15]  Hyun Seok Song,et al.  Single‐Carbon‐Atomic‐Resolution Detection of Odorant Molecules using a Human Olfactory Receptor‐based Bioelectronic Nose , 2009 .

[16]  Min Yang,et al.  Earthy odor compounds production and loss in three cyanobacterial cultures. , 2012, Water research.

[17]  Tai Hyun Park,et al.  Nanovesicle-based bioelectronic nose platform mimicking human olfactory signal transduction. , 2012, Biosensors & bioelectronics.

[18]  S. Watson,et al.  Quantitative analysis of trace levels of geosmin and MIB in source and drinking water using headspace SPME , 2000 .

[19]  Tai Hyun Park,et al.  A bioelectronic sensor based on canine olfactory nanovesicle-carbon nanotube hybrid structures for the fast assessment of food quality. , 2012, The Analyst.

[20]  Tai Hyun Park,et al.  Recent advances in electronic and bioelectronic noses and their biomedical applications. , 2011, Enzyme and microbial technology.

[21]  Manuel J. Rodríguez,et al.  Influence of water quality on the presence of off-flavour compounds (geosmin and 2-methylisoborneol). , 2010, Water research.

[22]  Hanns Hatt,et al.  Specificity and Sensitivity of a Human Olfactory Receptor Functionally Expressed in Human Embryonic Kidney 293 Cells andXenopus Laevis Oocytes , 1999, The Journal of Neuroscience.

[23]  Tai Hyun Park,et al.  Nanovesicle‐Based Bioelectronic Nose for the Diagnosis of Lung Cancer from Human Blood , 2014, Advanced healthcare materials.

[24]  L. Buck,et al.  Combinatorial Receptor Codes for Odors , 1999, Cell.

[25]  Hwi Jin Ko,et al.  Dual signal transduction mediated by a single type of olfactory receptor expressed in a heterologous system , 2006, Biological chemistry.

[26]  S. Watson,et al.  Actinomycetes in relation to taste and odour in drinking water: myths, tenets and truths. , 2006, Water research.