CLOSTRIDIUM RUBRUM SP. N. AND OTHER PECTINOLYTIC CLOSTRIDIA FROM SOIL

Ng, Henry (University of California, Davis) and Reese H. Vaughn. Clostridium rubrum sp. n. and other pectinolytic clostridia from soil. J. Bacteriol. 85:1104–1113. 1963.—Reports in the literature and results of experiments described herein suggest that pectinolytic anaerobes constitute a very heterogeneous group. The cultures isolated in this study all belonged to the genus Clostridium. The following species were identified: C. butyricum, C. fallax, C. multifermentans, and C. indolis. In addition, a species believed to be previously undescribed was named C. rubrum sp. n. The ability to ferment galacturonic acid was found to be adaptive. Some cultures fermented pectin and pectic acid to the same degree, whereas others fermented pectin only partially. The partial fermentation was attributed to the lack of a pectinesterase. On the basis of fermentation balances, it was concluded that the four strains of galacturonic acid fermenters selected for study yielded identical end products in approximately the same proportions. Per mole of galacturonic acid fermented, about 2 moles of CO2, 1.5 moles of H2, 1.5 moles of acetic acid, and 0.25 mole of butyric acid were produced.

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