Many scholars have attempted to use new methods inspired by DNA bio-techniques in the domains of cryptography and steganography. In this article, a new S-Box was designed inspired by biology DNA techniques to be used for SPN symmetric block ciphers. The new S-Box is used in order to make use of biological process as inspiration in creating the S-Box as simple and secure approach. This article uses the new S-Box within the AES (Advanced Encryption Standard) .The National Institute of Standard and Technology (NIST) tests have been used to test the cipher which uses this new S-Box. The results of the tests demonstrate that it effectively passed all the randomness tests of NIST. In addition, the S-Box testing criteria were conducted to test the security of the new S-Box; the results of these tests indicate that the new S-Box has good security.