Profiles of alkaline phosphatase isoenzymes in serum using cellulose acetate electrophoresis and organ-specific inhibitors.

Profiles of isoenzymes of alkaline phosphatase in the serum using a substrate-gel imprint following electrophoresis on cellulose acetate, organ-specific inhibitors (urea and L-phenylalanine), and heat inactivation are presented. The behavior of the frequently encountered mixtures of isoenzymes is emphasized. The isoenzyme couplet seen in hepatobiliary disorders is studied in relation to magnitude of total alkaline phosphatase, ABO blood group, and serum bilirubin levels. The alkaline phosphatase electrophoretic patterns seen in the sera of 43 normal pregnant women at term are classified according to the amount of placental alkaline phosphatase present and are related to age, blood group, and multiparity.