Characterization in vitro and in vivo of the oxygen dependence of an enzyme/polymer biosensor for monitoring brain glucose

The oxygen dependence of a first generation amperometric biosensor was investigated in vitro and in vivo by monitoring its glucose response as a function of solution pO(2). The biosensor was a glucose oxidase (GOx) modified poly(o-phenylenediamine) coated Pt cylinder electrode (Pt/PPD/GOx) that has been designed for neurochemical analysis in vivo. Two types of oxygen probes were used: a self-calibrating commercial macroelectrode in vitro; and a carbon paste microelectrode in vivo. Calibrations in vitro showed that oxygen interference in the operation of Pt/PPD/GOx electrodes was minimal for concentrations of glucose (approximately 0.5 mM) and oxygen (approximately 50 microM) found in brain ECF. This observation was confirmed by simultaneous monitoring in vivo of brain glucose and oxygen in the awake rat. However, at levels of glucose normally found in peripheral tissues (approximately 5 mM), the oxygen dependence was severe. We conclude that the oxygen sensitivity of Pt/PPD/GOx biosensors does not preclude their reliable use in media containing low glucose levels, such as brain ECF.

[1]  G. Rivas,et al.  Iridium‐dispersed carbon paste enzyme electrodes , 1996 .

[2]  J. Lowry,et al.  Characterization of Glucose Oxidase-Modified Poly(phenylenediamine)-Coated Electrodes in vitro and in vivo: Homogeneous Interference by Ascorbic Acid in Hydrogen Peroxide Detection , 1994 .

[3]  V. Yang,et al.  Biomedical application of immobilized enzymes. , 2000, Journal of pharmaceutical sciences.

[4]  E. Hall,et al.  Model for an immobilized oxidase enzyme electrode in the presence of two oxidants , 1994 .

[5]  G. S. Wilson,et al.  In vitro and in vivo evaluation of oxygen effects on a glucose oxidase based implantable glucose sensor , 1993 .

[6]  C. Malitesta,et al.  Glucose fast-response amperometric sensor based on glucose oxidase immobilized in an electropolymerized poly(o-phenylenediamine) film. , 1990, Analytical chemistry.

[7]  M. Fillenz,et al.  Real-time monitoring of brain energy metabolism in vivo using microelectrochemical sensors: the effects of anesthesia. , 2001, Bioelectrochemistry.

[8]  R. O'Neill,et al.  Major differences in the behaviour of carbon paste and carbon fibre electrodes in a protein-lipid matrix: implications for voltammetry in vivo. , 1998, The Analyst.

[9]  Martyn G Boutelle,et al.  Measurement of brain tissue oxygen at a carbon paste electrode can serve as an index of increases in regional cerebral blood flow , 1997, Journal of Neuroscience Methods.

[10]  N. Sakamoto.  Effect of enzyme concentration on the dynamic behavior of a membrane-bound enzyme system , 1992 .

[11]  D. Gough,et al.  Transient response of the two-dimensional glucose sensor. , 1988, Analytical chemistry.

[12]  Studies of a glassy carbon electrode for brain polarography with observations on the effect of carbonic anhydrase inhibition. , 1965, The Alabama journal of medical sciences.

[13]  A Heller,et al.  Amperometric glucose microelectrodes prepared through immobilization of glucose oxidase in redox hydrogels. , 1991, Analytical chemistry.

[14]  Joseph Wang,et al.  Glucose Biosensors : 40 Years of Advances and Challenges , 2001 .

[15]  A novel, remote-controlled suspension device for brain tissue PO2 measurements with multiwire surface electrodes , 1994, Pflügers Archiv.

[16]  J. Eyre,et al.  Glucose export from the brain in man: evidence for a role for astrocytic glycogen as a reservoir of glucose for neural metabolism , 1994, Brain Research.

[17]  S Zhang,et al.  Materials and techniques for electrochemical biosensor design and construction. , 2000, Biosensors & bioelectronics.

[18]  P. Bartlett,et al.  A Microelectrochemical Enzyme Transistor Responsive to Glucose , 1994 .

[19]  D. Wilke,et al.  Preparation and analytical testing of mediator containing photolithographically patterned enzyme membrane electrodes , 1994 .

[20]  G. Hicks,et al.  The Enzyme Electrode , 1967, Nature.

[21]  D. Buerk,et al.  Comparisons of oxygen metabolism and tissue PO2 in cortex and hippocampus of gerbil brain. , 1987, Stroke.

[22]  B. Siesjö,et al.  Brain energy metabolism , 1978 .

[23]  Robert D. O'Neill,et al.  Efficient glucose detection in anaerobic solutions using an enzyme-modified electrode designed to detect H2O2: implications for biomedical applications , 1994 .

[24]  C. M. Braguglia.  Biosensors : An outline of general principles and application , 1998 .

[25]  Joseph Wang,et al.  Oxygen-Rich Oxidase Enzyme Electrodes for Operation in Oxygen-Free Solutions , 1998 .

[26]  R. O'Neill.  Sensor-tissue interactions in neurochemical analysis with carbon paste electrodes in vivo. , 1993, The Analyst.

[27]  Philip N. Bartlett,et al.  Electrochemical immobilisation of enzymes: Part I. Theory , 1987 .

[28]  M. Fillenz,et al.  Evidence for uncoupling of oxygen and glucose utilization during neuronal activation in rat striatum. , 1997, The Journal of physiology.

[29]  W. Göpel,et al.  Monoalkylferrocene-mediated amperometric enzyme electrodes for glucose determination , 1993 .

[30]  M. Mas,et al.  Monitoring brain chemistry in vivo: voltammetric techniques, sensors, and behavioral applications. , 1998, Critical reviews in neurobiology.

[31]  P. E. Gold,et al.  Fluctuations in Brain Glucose Concentration during Behavioral Testing: Dissociations between Brain Areas and between Brain and Blood , 2001, Neurobiology of Learning and Memory.

[32]  Zhiqiang Gao,et al.  Electrocatalysis and flow-injection analysis of hydrogen peroxide at a chemically modified electrode , 1992 .

[33]  H. Hill,et al.  Homogeneous ferrocene-mediated amperometric immunoassay. , 1986, Analytical chemistry.

[34]  M. Fillenz,et al.  Characterization of carbon paste electrodes in vitro for simultaneous amperometric measurement of changes in oxygen and ascorbic acid concentrations in vivo. , 1996, The Analyst.

[35]  M. Fillenz,et al.  Extracellular glucose turnover in the striatum of unanaesthetized rats measured by quantitative microdialysis , 1997, The Journal of physiology.

[36]  Daniel Thomas,et al.  Electrochemical study of d-glucose oxidase autoinactivation , 1982 .

[37]  E. Magner,et al.  Trends in electrochemical biosensors. , 1998, The Analyst.

[38]  Babu Subramanyam,et al.  MONITORING MOLECULES IN NEUROSCIENCE , 1991 .

[39]  Alexander M. Yacynych,et al.  Electropolymerized 1,2-diaminobenzene as a means to prevent interferences and fouling and to stabilize immobilized enzyme in electrochemical biosensors , 1990 .

[40]  M. Fillenz,et al.  Continuous Monitoring of Extracellular Glucose Concentrations in the Striatum of Freely Moving Rats with an Implanted Glucose Biosensor , 1998, Journal of neurochemistry.

[41]  E. R. Reynolds,et al.  Platinized carbon ultramicroelectrodes as glucose biosensors , 1993 .

[42]  L. C. Clark,et al.  ELECTRODE SYSTEMS FOR CONTINUOUS MONITORING IN CARDIOVASCULAR SURGERY , 1962 .

[43]  J Wang,et al.  Electroanalysis and biosensors. , 1993, Analytical chemistry.

[44]  Martyn G Boutelle,et al.  An amperometric glucose-oxidase/poly(o-phenylenediamine) biosensor for monitoring brain extracellular glucose: in vivo characterisation in the striatum of freely-moving rats , 1998, Journal of Neuroscience Methods.

[45]  Isao Karube,et al.  Enzyme sensors for environmental analysis , 2000 .

[46]  A. Heller,et al.  Hydrogen peroxide and .beta.-nicotinamide adenine dinucleotide sensing amperometric electrodes based on electrical connection of horseradish peroxidase redox centers to electrodes through a three-dimensional electron relaying polymer network , 1992 .

[47]  Joseph Wang,et al.  Highly Selective Membrane-Free, Mediator-Free Glucose Biosensor , 1994 .