X-ray microtomography to study the microstructure of cream cheese-type products.

In this work, the imaging x-ray microtomography technique, new to the field of food science, was used for the analysis of fat microstructure and quantification of the fat present in cream cheese-type products. Five different types of commercially produced cheeses, chosen for their variability of texture, were used for this experiment: sample A, sample B, sample C, sample D, and sample E. Appropriate quantitative 3-dimensional parameters describing the fat structure were calculated (e.g., the geometric parameter percentage of fat volume was calculated for each image as a representation of the percentage of total fat content within the sample). The dynamic-mechanical properties of these samples were also studied using a controlled-strain rotational rheometer. Storage modulus and loss modulus were determined in a frequency range of 0.01 to 10 Hz. The strain value was obtained by preliminary strain sweep oscillatory trials to determine the linear viscoelastic region of the cream cheese-type products. Statistical correlation analysis was performed on the results to help identify any microstructural-mechanical structure relationships. The results from this study show that microtomography is a suitable technique for the microstructural analysis of fat in cream cheese-type products, as it does not only provide an accurate percentage of the volume of the fat present but can also determine its spatial distribution.

[1]  J. Steffe,et al.  Texture of Cheddar Cheese as Influenced by Fat Reduction , 1995 .

[2]  L. Bohlin A theory of flow as a cooperative phenomenon , 1980 .

[3]  Michael H. Tunick,et al.  Chemistry of Structure-Function Relationships in Cheese , 2012, Advances in Experimental Medicine and Biology.

[4]  M. Kaláb,et al.  Milk gel structure. XV. Electron microscopy of whey protein-based cream cheese spread , 1985 .

[5]  P. Falcone,et al.  A novel approach to the study of bread porous structure: Phase-contrast X-ray microtomography , 2004 .

[6]  Thomas A. Albert,et al.  Threshold selection using a minimal histogram entropy difference , 1997 .

[7]  H. K. Mebatsion,et al.  Three-dimensional pore space quantification of apple tissue using X-ray computed microtomography , 2007, Planta.

[8]  B. Bergenståhl,et al.  Low-fat mayonnaise:: Influences of fat content, aroma compounds and thickeners , 1997 .

[9]  V. V. Mistry,et al.  Composition and Microstructure of Commercial Full-Fat and Low-Fat Cheeses , 1993 .

[10]  M Vogel,et al.  Trabecular bone pattern factor--a new parameter for simple quantification of bone microarchitecture. , 1992, Bone.

[11]  Confocal scanning laser microscopy and quantitative image analysis: application to cream cheese microstructure investigation. , 2008, Journal of dairy science.

[12]  M. Kaláb Practical Aspects of Electron Microscopy in Dairy Research , 1993 .

[13]  K. S. Lim,et al.  X-ray micro-computed tomography of cellular food products , 2004 .

[14]  M. A. Rao,et al.  Viscoelastic properties of foods , 1992 .

[15]  K. Wendin,et al.  Flavour and texture in sourmilk affected by thickeners and fat content , 1997 .

[16]  P. Fryer,et al.  A Novel Technique for Ice Crystal Visualization in Frozen Solids Using X‐Ray Micro‐Computed Tomography , 2005 .

[17]  K. Wendin,et al.  Dynamic analyses of sensory and microstructural properties of cream cheese , 2000 .

[18]  N. Desai,et al.  Microstructure studies of reduced-fat cheese containing fat substitute. , 1995, Advances in experimental medicine and biology.

[19]  D. Peressini,et al.  Rheological characterization of traditional and light mayonnaises , 1998 .

[20]  Sakamon Devahastin,et al.  Effect of far-infrared radiation assisted drying on microstructure of banana slices: An illustrative use of X-ray microtomography in microstructural evaluation of a food product , 2008 .

[21]  E. Dickinson,et al.  Food Emulsions and Foams: Interfaces, Interactions and Stability , 1999 .

[22]  S. Gunasekaran,et al.  SMALL AMPLITUDE OSCILLATORY SHEAR STUDIES ON MOZZARELLA CHEESE PART I. REGION OF LINEAR VISCOELASTICITY , 1997 .

[23]  Lucia Mancini,et al.  Three-dimensional quantitative analysis of bread crumb by X-ray microtomography. , 2006 .

[24]  Martine Wevers,et al.  The role of sugar and fat in sugar-snap cookies: Structural and textural properties , 2009 .

[25]  E. Dickinson,et al.  Food Colloids, Proteins, Lipids and Polysaccharides , 1997 .

[26]  P. Rüegsegger,et al.  A new method for the model‐independent assessment of thickness in three‐dimensional images , 1997 .

[27]  Martin G. Scanlon,et al.  The bubble size distribution in wheat flour dough , 2006 .