Determination of zearalenone in barley by high-performance liquid chromatography coupled with evaporative light scattering detection and natural occurrence of zearalenone in functional food

Abstract Zearalenone (ZEA) is one of the most widely distributed mycotoxins produced by Fusarium species, responsible for important diseases of barley. The present work shows the results of a rapid and accurate method to quantify ZEA in barley. The method uses the Quick Easy Cheap Effective Rugged and Safe method (QuEChERS) for purification and high-performance liquid chromatography coupled with an evaporative laser scattering detector (HPLC–ELSD) for detection and quantification of the toxin. Barley samples were extracted with methanol and used acetonitrile–water–methanol (46:46:8, v/v) as mobile phase. Average recoveries of ZEA from barley spiked at levels of 0.1–10 μg/g ranged from 83.0% to 91.5%, with relative standard deviations of

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