Role Of Interleukin-8 In Periodontal Disease

AIM & OBJECTIVE: a) To estimate the concentration of IL-8 in health, in plaque associated gingivitis and in chronic periodontitis.b) To correlate the concentration of IL-8 with other clinical parameters of gingival and periodontal status.c)To determine if IL-8 can act as a marker/indicator in chronic periodontal disease destruction. STUDY DESIGN: A total of 75 systemically healthy patients, aged 25-50 years were included in the study. Based on the clinical evaluation and radiographic examination, the patients were divided into 3 groups - healthy, plaque associated gingivitis and chronic periodontitis. Gingival Crevicular fluid from these patients was collected using micro-capillary pipettes and the concentrations of IL-8 in the samples were determined using ELISA. RESULTS: IL-8 was detected in all three groups. In periodontally diseased subjects, the concentration of IL-8 was significantly elevated when compared to healthy subjects. CONCLUSION: The results suggested that gingival crevicular IL-8 levels increases with the increase in periodontal destruction. Based on the results of this study and previous clinical trials this article discusses the relevance of IL-8 in chronic periodontitis and its role as a biological marker.

[1]  J. Gamonal,et al.  Stimulatory response of neutrophils from periodontitis patients with periodontal pathogens. , 2007, Oral diseases.

[2]  S. Socransky,et al.  Introduction to microbial aspects of periodontal biofilm communities, development and treatment. , 2006, Periodontology 2000.

[3]  M. C. Santos,et al.  Genetic polymorphisms in the MMP-1 and MMP-3 gene may contribute to chronic periodontitis in a Brazilian population. , 2006, Journal of clinical periodontology.

[4]  M. Rouabhia,et al.  Porphyromonas gingivalis-Epithelial Cell Interactions in Periodontitis , 2006, Journal of dental research.

[5]  R. Darveau,et al.  Modulation of the innate immune response within the periodontium. , 2004, Periodontology 2000.

[6]  A. Mombelli,et al.  Effect of inflammation, smoking and stress on gingival crevicular fluid cytokine level. , 2003, Journal of clinical periodontology.

[7]  A. Kurdowska,et al.  Interleukin-8 and anti-interleukin-8 autoantibodies in gingival crevicular fluid from patients with periodontitis. , 2003, Journal of periodontal research.

[8]  W. K. Leung,et al.  Relationship of changes in interleukin-8 levels and granulocyte elastase activity in gingival crevicular fluid to subgingival periodontopathogens following non-surgical periodontal therapy in subjects with chronic periodontitis. , 2002, Journal of clinical periodontology.

[9]  A. Gustafsson,et al.  Increased amounts of laminin in GCF from untreated patients with periodontitis. , 2000, Journal of clinical periodontology.

[10]  N. Lang,et al.  Neutrophil migration into the gingival sulcus is associated with transepithelial gradients of interleukin-8 and ICAM-1. , 1998, Journal of periodontology.

[11]  J. Ebersole,et al.  Increased presence of interleukin-6 (IL-6) and IL-8 secreting fibroblast subpopulations in adult periodontitis. , 1998, Journal of periodontology.

[12]  R. Page,et al.  The microbial challenge in periodontitis. , 1997, Periodontology 2000.

[13]  R. Page,et al.  The pathogenesis of human periodontitis: an introduction. , 1997, Periodontology 2000.

[14]  I. Lamster,et al.  Interleukin-8 and beta-glucuronidase in gingival crevicular fluid. , 1997, Journal of clinical periodontology.

[15]  D. Taub.  Chemokine-leukocyte interactions. The voodoo that they do so well. , 1996, Cytokine & growth factor reviews.

[16]  D. Aeppli,et al.  Interleukin-1 alpha, interleukin-8 and interferon-alpha levels in gingival crevicular fluid. , 1996, Journal of periodontal research.

[17]  C. Tsai,et al.  Levels of interleukin-1 beta and interleukin-8 in gingival crevicular fluids in adult periodontitis. , 1995, Journal of periodontology.

[18]  N. Lang,et al.  Detection of interleukin-8 and matrix metalloproteinases transcripts in healthy and diseased gingival biopsies by RNA/PCR. , 1993, Journal of periodontal research.

[19]  B. Dewald,et al.  Enhanced production of neutrophil-activating peptide-1/interleukin-8 in rheumatoid arthritis. , 1991, The Journal of clinical investigation.

[20]  J. Sterne,et al.  Detection of high-risk groups and individuals for periodontal diseases: laboratory markers from analysis of saliva. , 1989, Journal of clinical periodontology.

[21]  S. Offenbacher,et al.  The use of crevicular fluid prostaglandin E2 levels as a predictor of periodontal attachment loss. , 1986, Journal of periodontal research.

[22]  D. Carnes,et al.  Determination of periodontal disease activity by a bone resorption assay. , 1984, Journal of periodontal research.

[23]  S. Socransky,et al.  Clinical parameters as predictors of destructive periodontal disease activity. , 1983, Journal of clinical periodontology.

[24]  F. Dewhirst,et al.  Levels of prostaglandin E2, thromboxane, and prostacyclin in periodontal tissues. , 1983, Journal of periodontal research.

[25]  H. Goldman,et al.  The role of endotoxin in periodontal disease II. Correlation of the quantity of endotoxin in human gingival exudate with the clinical degree of inflammation. , 1970, Journal of periodontology.

[26]  G. Cimasoni,et al.  Collection of Gingival Fluid for Quantitative Analysis , 1969, Journal of dental research.

[27]  B. Åsman,et al.  IL-8 and TNF-alpha from peripheral neutrophils and acute-phase proteins in periodontitis. , 2002, Journal of clinical periodontology.

[28]  S. Murakami,et al.  Cytokine expression in periodontal health and disease. , 1998, Critical reviews in oral biology and medicine : an official publication of the American Association of Oral Biologists.