Coxsackievirus and Adenovirus Receptor Amino-Terminal Immunoglobulin V-Related Domain Binds Adenovirus Type 2 and Fiber Knob from Adenovirus Type 12

ABSTRACT The extracellular region of the coxsackievirus and adenovirus receptor (CAR) is predicted to consist of two immunoglobulin (Ig)-related structural domains. We expressed the isolated CAR amino-terminal domain (D1) and a CAR fragment containing both extracellular Ig domains (D1/D2) in Escherichia coli. Both D1 and D1/D2 formed complexes in vitro with the recombinant knob domain of adenovirus type 12 (Ad12) fiber, and D1 inhibited adenovirus type 2 (Ad2) infection of HeLa cells. These results indicate that the adenovirus-binding activity of CAR is localized in the amino-terminal IgV-related domain and confirm our earlier observation that Ad2 and Ad12 bind to the same cellular receptor. Preliminary crystallization studies suggest that complexes of Ad12 knob bound to D1 will be suitable for structure determination.

[1]  David C. Joy,et al.  Introduction to analytical electron microscopy , 1979 .

[2]  B. Harfe,et al.  Mutations that alter an Arg-Gly-Asp (RGD) sequence in the adenovirus type 2 penton base protein abolish its cell-rounding activity and delay virus reproduction in flat cells , 1993, Journal of virology.

[3]  J. Bergelson,et al.  The Murine CAR Homolog Is a Receptor for Coxsackie B Viruses and Adenoviruses , 1998, Journal of Virology.

[4]  J. Deisenhofer,et al.  Characterization of the knob domain of the adenovirus type 5 fiber protein expressed in Escherichia coli , 1994, Journal of virology.

[5]  L. Philipson,et al.  HCAR and MCAR: the human and mouse cellular receptors for subgroup C adenoviruses and group B coxsackieviruses. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[6]  P. Boulanger,et al.  Human adenovirus-host cell interactions: comparative study with members of subgroups B and C , 1990, Journal of virology.

[7]  L. Campisi,et al.  Vitronectin receptor antibodies inhibit infection of HeLa and A549 cells by adenovirus type 12 but not by adenovirus type 2 , 1994, Journal of virology.

[8]  J Deisenhofer,et al.  Crystal structure of the receptor-binding domain of adenovirus type 5 fiber protein at 1.7 A resolution. , 1994, Structure.

[9]  R. Crystal,et al.  Administration of an adenovirus containing the human CFTR cDNA to the respiratory tract of individuals with cystic fibrosis , 1994, Nature Genetics.

[10]  G. Nemerow,et al.  Integrins α v β 3 and α v β 5 promote adenovirus internalization but not virus attachment , 1993, Cell.

[11]  J F Hainfeld,et al.  Mass mapping with the scanning transmission electron microscope. , 1986, Annual review of biophysics and biophysical chemistry.

[12]  G. Mayr,et al.  A single locus on human chromosome 21 directs the expression of a receptor for adenovirus type 2 in mouse A9 cells , 1997, Journal of virology.

[13]  D. Staunton,et al.  A cell adhesion molecule, ICAM-1, is the major surface receptor for rhinoviruses , 1989, Cell.

[14]  J. Wall,et al.  Biological Scanning Transmission Electron Microscopy , 1979 .

[15]  J. Zabner,et al.  Lack of high affinity fiber receptor activity explains the resistance of ciliated airway epithelia to adenovirus infection. , 1997, The Journal of clinical investigation.

[16]  L. Philipson,et al.  Unrelated animal viruses share receptors , 1976, Nature.

[17]  P Bork,et al.  The immunoglobulin fold. Structural classification, sequence patterns and common core. , 1994, Journal of molecular biology.

[18]  G. Wadell,et al.  Molecular determinants of adenovirus tropism. , 1995, Current topics in microbiology and immunology.

[19]  E. Wimmer,et al.  Cellular receptor for poliovirus: Molecular cloning, nucleotide sequence, and expression of a new member of the immunoglobulin superfamily , 1989, Cell.

[20]  W. Schleif,et al.  A monoclonal antibody specific for the cellular receptor for the group B coxsackieviruses , 1988, Journal of virology.

[21]  R. Gibson,et al.  Repeat administration of an adenovirus vector encoding cystic fibrosis transmembrane conductance regulator to the nasal epithelium of patients with cystic fibrosis. , 1996, The Journal of clinical investigation.

[22]  M G Rossmann,et al.  Structure of a human rhinovirus complexed with its receptor molecule. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[23]  M. Rossmann,et al.  The structure of the two amino-terminal domains of human ICAM-1 suggests how it functions as a rhinovirus receptor and as an LFA-1 integrin ligand. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[24]  J. Deisenhofer,et al.  Structure of the receptor binding domain of adenovirus type 5 fiber protein. , 1995, Current topics in microbiology and immunology.

[25]  P. Hu,et al.  A controlled study of adenoviral-vector-mediated gene transfer in the nasal epithelium of patients with cystic fibrosis. , 1995, The New England journal of medicine.

[26]  T. Springer,et al.  A dimeric crystal structure for the N-terminal two domains of intercellular adhesion molecule-1. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[27]  Robin A. Weiss,et al.  The T4 gene encodes the AIDS virus receptor and is expressed in the immune system and the brain , 1986, Cell.

[28]  Joseph Zabner,et al.  Adenovirus-mediated gene transfer transiently corrects the chloride transport defect in nasal epithelia of patients with cystic fibrosis , 1993, Cell.

[29]  J. Melnick.  My role in the discovery and classification of the enteroviruses. , 1996, Annual review of microbiology.

[30]  P. Freimuth.  A human cell line selected for resistance to adenovirus infection has reduced levels of the virus receptor , 1996, Journal of virology.

[31]  R. Crystal,et al.  Modification of nasal epithelial potential differences of individuals with cystic fibrosis consequent to local administration of a normal CFTR cDNA adenovirus gene transfer vector. , 1995, Human gene therapy.

[32]  C. Pace,et al.  How to measure and predict the molar absorption coefficient of a protein , 1995, Protein science : a publication of the Protein Society.

[33]  U. Pettersson,et al.  Virus-Receptor Interaction in an Adenovirus System , 1968, Journal of virology.

[34]  J. Bergelson,et al.  Isolation of a Common Receptor for Coxsackie B Viruses and Adenoviruses 2 and 5 , 1997, Science.