Magnetic resonance microimaging for noninvasive quantification of myocardial function and mass in the mouse

The purpose of this work was to develop high‐resolution cardiac magnetic resonance imaging techniques for the in vivo mouse model for quantification of myocardial function and mass. Eight male mice were investigated on a 7‐Tesla MRI scanner. High‐quality images in multiple short axis slices (in‐plane resolution 117 μm2, slice thickness 1 mm) were acquired with an ECG‐gated cine sequence. Left ventricular end‐diastolic and end‐systolic volumes and mass were calculated from segmented slice volumes. There was precise agreement of left ventricular mass determined ex vivo and by MRI. lntraobserver (5%) and interobserver (5%) variability of in vivo MR measurements were low.

[1]  J. Gardin,et al.  Echocardiographic assessment of left ventricular mass and systolic function in mice. , 1995, Circulation research.

[2]  S. Rose,et al.  High resolution high field rodent cardiac imaging with flow enhancement suppression. , 1994, Magnetic resonance imaging.

[3]  R. Walsh,et al.  In vivo echocardiographic detection of enhanced left ventricular function in gene-targeted mice with phospholamban deficiency. , 1995, Circulation research.

[4]  D S Berman,et al.  Noninvasive quantification of left ventricular myocardial mass by gated proton nuclear magnetic resonance imaging. , 1987, Journal of the American College of Cardiology.

[5]  R. Weisskoff,et al.  Measurement of stroke volume and cardiac output within a single breath hold with echo‐planar MR imaging , 1994, Journal of magnetic resonance imaging : JMRI.

[6]  D. Altman,et al.  STATISTICAL METHODS FOR ASSESSING AGREEMENT BETWEEN TWO METHODS OF CLINICAL MEASUREMENT , 1986, The Lancet.

[7]  J Ross,et al.  Molecular and physiological alterations in murine ventricular dysfunction. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[8]  C. Higgins,et al.  Quantification of the left ventricular volumes and function with cine MR imaging: comparison of geometric models with three-dimensional data. , 1993, Radiology.

[9]  R Beyar,et al.  Determination of left ventricular mass by magnetic resonance imaging in hearts deformed by acute infarction. , 1989, Circulation.

[10]  A. Koretsky,et al.  Dilated Cardiomyopathy in Transgenic Mice With Cardiac-Specific Overexpression of Tumor Necrosis Factor-α , 1997 .

[11]  G M Pohost,et al.  Left ventricular volume measurement using cardiac axis nuclear magnetic resonance imaging. Validation by calibrated ventricular angiography. , 1990, Circulation.

[12]  L. Field.  Transgenic mice in cardiovascular research. , 1993, Annual review of physiology.

[13]  D. Firmin,et al.  Technical note: rapid measurement of left ventricular mass by spin echo magnetic resonance imaging. , 1994, The British journal of radiology.

[14]  C. Higgins,et al.  Interstudy reproducibility of dimensional and functional measurements between cine magnetic resonance studies in the morphologically abnormal left ventricle. , 1990, American heart journal.

[15]  J C Ehrhardt,et al.  Measurement of left ventricular mass in vivo using gated nuclear magnetic resonance imaging. , 1986, Journal of the American College of Cardiology.