Revised Sequence and Activity of Bacillus stearothermophilus Catalase I (Formerly Peroxidase)

Abstract The revised nucleotide sequence of the perA gene, encoding catalase I (formerly peroxidase) of Bacillus stearothermophilus , is presented. The deduced amino acid sequence was significantly similar to those of Escherichia coli catalase HPI, Salmonella typhimurium catalase HPI, and yeast cytochrome c peroxidase. Catalase I was purified and the ratio of the catalatic to peroxidatic activity was measured and found to be 654 to 1.

[1]  Hans Ulrich Bergmeyer,et al.  Methods of Enzymatic Analysis , 2019 .

[2]  H. Okada,et al.  Cloning, nucleotide sequence, and expression in Escherichia coli of the Bacillus stearothermophilus peroxidase gene (perA) , 1989, Journal of bacteriology.

[3]  P. Loewen,et al.  Physical characterization of katG, encoding catalase HPI of Escherichia coli. , 1987, Gene.

[4]  I. Fridovich,et al.  Purification of the o-dianisidine peroxidase from Escherichia coli B. Physicochemical characterization and analysis of its dual catalatic and peroxidatic activities. , 1979, The Journal of biological chemistry.

[5]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[6]  F. Sanger,et al.  DNA sequencing with chain-terminating inhibitors. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[7]  H. Okada,et al.  Thermostable peroxidase from Bacillus stearothermophilus. , 1988, Journal of general microbiology.

[8]  K. Welinder.  Bacterial catalase-peroxidases are gene duplicated members of the plant peroxidase superfamily. , 1991, Biochimica et biophysica acta.

[9]  A. G. Hildebraunt,et al.  Reduced nicotinamide adenine dinucleotide phosphate (NADPH)-dependent formation and breakdown of hydrogen peroxide during mixed function oxidation reactions in liver microsomes. , 1975, Archives of biochemistry and biophysics.

[10]  B. Doble,et al.  Nucleotide sequence of katG, encoding catalase HPI of Escherichia coli , 1988, Journal of bacteriology.

[11]  D. Lipman,et al.  Improved tools for biological sequence comparison. , 1988, Proceedings of the National Academy of Sciences of the United States of America.