Speed breeding in growth chambers and glasshouses for crop breeding and model plant research

Abstract‘Speed breeding’ (SB) shortens the breeding cycle and accelerates crop research through rapid generation advancement. SB can be carried out in numerous ways, one of which involves extending the duration of plants’ daily exposure to light, combined with early seed harvest, to cycle quickly from seed to seed, thereby reducing the generation times for some long-day (LD) or day-neutral crops. In this protocol, we present glasshouse and growth chamber–based SB approaches with supporting data from experimentation with several crops. We describe the conditions that promote the rapid growth of bread wheat, durum wheat, barley, oat, various Brassica species, chickpea, pea, grass pea, quinoa and Brachypodium distachyon. Points of flexibility within the protocols are highlighted, including how plant density can be increased to efficiently scale up plant numbers for single-seed descent (SSD). In addition, instructions are provided on how to perform SB on a small scale in a benchtop growth cabinet, enabling optimization of parameters at a low cost.This protocol describes procedures for speed-breeding approaches using growth cabinets and LED-supplemented glasshouses. The approaches can be used to accelerate crop research and are compatible with a wide variety of crops.

[1]  K. Nelson,et al.  Precocious floral initiation and identification of exact timing of embryo physiological maturity facilitate germination of immature seeds to truncate the lifecycle of pea , 2017, Plant Growth Regulation.

[2]  V. Sosa‐Zuniga,et al.  Phenological growth stages of quinoa (Chenopodium quinoa) based on the BBCH scale , 2017 .

[3]  S. Bhattarai,et al.  In vitro culture of immature seed for rapid generation advancement in tomato , 2009, Euphytica.

[4]  Guiyin Zhang,et al.  An integrated breeding technology for accelerating generation advancement and trait introgression in cotton , 2011 .

[5]  R. K. Meyer,et al.  Design and Construction of an Inexpensive Homemade Plant Growth Chamber , 2015, PloS one.

[6]  I. DeLacy,et al.  Grain dormancy in fixed lines of white-grained wheat (Triticum aestivum L.) grown under controlled environmental conditions , 2009, Euphytica.

[7]  T. Warkentin,et al.  Plant growth regulators improve in vitro flowering and rapid generation advancement in lentil and faba bean , 2014, In Vitro Cellular & Developmental Biology - Plant.

[8]  K. Takeno,et al.  Stress-induced flowering , 2010, Plant signaling & behavior.

[9]  Rodomiro Ortiz,et al.  Haploids: Constraints and opportunities in plant breeding. , 2015, Biotechnology advances.

[10]  Lee Hickey,et al.  A rapid phenotyping method for adult plant resistance to leaf rust in wheat , 2016, Plant Methods.

[11]  T. Warkentin,et al.  Low red: Far-red light ratio causes faster in vitro flowering in lentil , 2016, Canadian Journal of Plant Science.

[12]  L. Evans.  Short Day Induction of Inflorescence Initiation in Some Winter Wheat Varieties , 1987 .

[13]  Peng Zhang,et al.  How to advance up to seven generations of canola (Brassica napus L.) per annum for the production of pure line populations? , 2016, Euphytica.

[14]  Z. Pretorius,et al.  An accelerated method for evaluating adult-plant resistance to leaf and stripe rust in spring wheat. , 2000 .

[15]  K. Schmid,et al.  Crossing Methods and Cultivation Conditions for Rapid Production of Segregating Populations in Three Grain Amaranth Species , 2016, Front. Plant Sci..

[16]  Gerald N. De La Fuente,et al.  Accelerating plant breeding. , 2013, Trends in plant science.

[17]  J. Croser,et al.  In vitro-assisted single-seed descent for breeding-cycle compression in subterranean clover (Trifolium subterraneum L.) , 2017, Crop and Pasture Science.

[18]  D. Jones,et al.  Responses of wheat to vernalization and photoperiod , 1985 .

[19]  Marina I. Sysoeva,et al.  Plants under Continuous Light: A Review , 2010 .

[20]  S. Hendricks,et al.  Photoperiodism in Plants. , 1960, Science.

[21]  W. Fehr,et al.  Stage of Development Descriptions for Soybeans, Glycine Max (L.) Merrill , 1971 .

[22]  Takeshi Hayashi,et al.  A practical, rapid generation-advancement system for rice breeding using simplified biotron breeding system , 2016, Breeding science.

[23]  M. Dieters,et al.  Rapid phenotyping for adult-plant resistance to stripe rust in wheat , 2012 .

[24]  Hui Liu,et al.  A fast generation cycling system for oat and triticale breeding , 2016 .

[25]  M. Dieters,et al.  Speed breeding for multiple disease resistance in barley , 2017, Euphytica.

[26]  D. Knott,et al.  Comparison of Early Generation Yield Testing and a Single Seed Descent Procedure in Wheat Breeding , 1975 .

[27]  M. Aydin,et al.  The role of growth regulators, embryo age and genotypes on immature embryo germination and rapid generation advancement in tomato ( Lycopersicon esculentum Mill.) , 2011 .

[28]  E. Yuliwati,et al.  A Review , 2019, Current Trends and Future Developments on (Bio-) Membranes.

[29]  R. Sylvester-Bradley,et al.  code for stages of development in oilseed rape (Brassica napus L.) , 1984 .

[30]  P. Finnegan,et al.  Antigibberellin-induced reduction of internode length favors in vitro flowering and seed-set in different pea genotypes , 2014, Biologia Plantarum.

[31]  J. Croser,et al.  In vitro reproduction in the annual pasture legumes subterranean clover (Trifolium subterraneum L.) and French serradella (Ornithopus sativus Brot.) , 2016 .

[32]  J. Batley,et al.  Speed breeding: a powerful tool to accelerate crop research and breeding , 2017, bioRxiv.

[33]  R. Creasy,et al.  Time to flowering of temperate pulses in vivo and generation turnover in vivo–in vitro of narrow-leaf lupin accelerated by low red to far-red ratio and high intensity in the far-red region , 2016, Plant Cell, Tissue and Organ Culture (PCTOC).

[34]  R M Wheeler,et al.  NASA's Biomass Production Chamber: a testbed for bioregenerative life support studies. , 1996, Advances in space research : the official journal of the Committee on Space Research.

[35]  S. Jackson.  Plant responses to photoperiod. , 2009, The New phytologist.

[36]  C. Bermejo,et al.  In vitro embryo culture to shorten the breeding cycle in lentil (Lens culinaris Medik) , 2016, Plant Cell Tissue and Organ Culture.

[37]  G. Gregorio,et al.  Revisiting rice breeding methods – evaluating the use of rapid generation advance (RGA) for routine rice breeding , 2017 .

[38]  R. Ortiz,et al.  High yield potential, shuttle breeding, genetic diversity, and a new international wheat improvement strategy , 2007, Euphytica.

[39]  T. Warkentin,et al.  Plant Tissue Culture , 1991, Bio/Technology.

[40]  G. Chen,et al.  A procedure allowing up to eight generations of wheat and nine generations of barley per annum , 2013, Euphytica.

[41]  J. Zadoks.  A decimal code for the growth stages of cereals , 1974 .

[42]  E. Heuvelink,et al.  A single locus confers tolerance to continuous light and allows substantial yield increase in tomato , 2014, Nature Communications.

[43]  F. Bassi,et al.  Speed breeding for multiple quantitative traits in durum wheat , 2018, Plant Methods.

[44]  L. Hickey,et al.  Resistance to yellow spot in wheat grown under accelerated growth conditions , 2016, Euphytica.

[45]  P. Roumet,et al.  Germination of immature soybean seeds to shorten reproductive cycle duration , 1997 .

[46]  P. Perney,et al.  New approaches towards the shortening of generation cycles for faster breeding of protein legumes , 2002 .

[47]  D. Braun,et al.  An Inexpensive, Easy-to-Use, and Highly Customizable Growth Chamber Optimized for Growing Large Plants. , 2017, Current protocols in plant biology.

[48]  Hui Liu,et al.  A fully in vitro protocol towards large scale production of recombinant inbred lines in wheat (Triticum aestivum L.) , 2017, Plant Cell, Tissue and Organ Culture (PCTOC).

[49]  D. L. George,et al.  Development and Application of Speed Breeding Technologies in a Commercial Peanut Breeding Program , 2013 .