High Pressure Liquid Chromatographic Assay of Cefamandole in Serum Following Intravenous and Intraperitoneal Administration

Abstract Following cesarean section 102 women were treated with cefamandole by either perioperative intravenous administration or intraperitoneal irrigation. High-pressure liquid chromatographic (HPLC) methods for the quantitation of the low serum levels of cefamandole following intraperitoneal lavage were developed. The antibiotic was assayed in the serum using a standard microbiological assay and two types of reverse phase column technology for HPLC. The two HPLC systems were almost identical in performance. Both HPLC methods were at least 10-fold more sensitive than the microbiological assay. The correlation between the three methods was 0.9739. The half-life of cefamandole was 37 min, which was not significantly different from the half-life of the drug in serum of non-pregnant women. The peak serum levels were 47.6 ± 36.8 μg/ml and 1.98 ± 1.5 μg/ml for the intravenous and intraperitoneal methods of administration, respectively.

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