Electroporation of mice zygotes with dual guide RNA/Cas9 complexes for simple and efficient cloning-free genome editing

[1]  Jennifer A. Doudna,et al.  Enhanced proofreading governs CRISPR-Cas9 targeting accuracy , 2017, Nature.

[2]  Y. Hérault,et al.  Modeling human disease in rodents by CRISPR/Cas9 genome editing , 2017, Mammalian Genome.

[3]  Channabasavaiah B. Gurumurthy,et al.  Simplified CRISPR tools for efficient genome editing and streamlined protocols for their delivery into mammalian cells and mouse zygotes , 2017, Methods.

[4]  Y. Yamashita,et al.  Electroporation of Cas9 protein/sgRNA into early pronuclear zygotes generates non-mosaic mutants in the mouse. , 2016, Developmental biology.

[5]  M. Matsuhisa,et al.  Somatic cell reprogramming-free generation of genetically modified pigs , 2016, Science Advances.

[6]  Ignacio Anegon,et al.  Comparative Analysis of piggyBac, CRISPR/Cas9 and TALEN Mediated BAC Transgenesis in the Zygote for the Generation of Humanized SIRPA Rats , 2016, Scientific Reports.

[7]  F. Flamant,et al.  CRISPR / Cas 9 : a breakthrough in generating mouse models for endocrinologists , 2016 .

[8]  J. Kent,et al.  Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection tool CRISPOR , 2016, Genome Biology.

[9]  F. W. Buaas,et al.  Delivery of Cas9 Protein into Mouse Zygotes through a Series of Electroporation Dramatically Increases the Efficiency of Model Creation. , 2016, Journal of genetics and genomics = Yi chuan xue bao.

[10]  Marc Tessier-Lavigne,et al.  Efficient introduction of specific homozygous and heterozygous mutations using CRISPR/Cas9 , 2016, Nature.

[11]  Lin He,et al.  Highly Efficient Mouse Genome Editing by CRISPR Ribonucleoprotein Electroporation of Zygotes* , 2016, The Journal of Biological Chemistry.

[12]  J. Keith Joung,et al.  731. High-Fidelity CRISPR-Cas9 Nucleases with No Detectable Genome-Wide Off-Target Effects , 2016 .

[13]  Jean-Paul Concordet,et al.  Improved Genome Editing Efficiency and Flexibility Using Modified Oligonucleotides with TALEN and CRISPR-Cas9 Nucleases. , 2016, Cell reports.

[14]  Li Zhang,et al.  Induction of site-specific chromosomal translocations in embryonic stem cells by CRISPR/Cas9 , 2016, Scientific Reports.

[15]  Adam Williams,et al.  Editing the Mouse Genome Using the CRISPR-Cas9 System. , 2016, Cold Spring Harbor protocols.

[16]  Jacob E Corn,et al.  Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA , 2016, Nature Biotechnology.

[17]  M. Ohtsuka,et al.  GONAD: A Novel CRISPR/Cas9 Genome Editing Method that Does Not Require Ex Vivo Handling of Embryos , 2016, Current protocols in human genetics.

[18]  David A. Scott,et al.  Rationally engineered Cas9 nucleases with improved specificity , 2015, Science.

[19]  C. Bennett,et al.  Synthetic CRISPR RNA-Cas9–guided genome editing in human cells , 2015, Proceedings of the National Academy of Sciences.

[20]  T. Mashimo,et al.  Simple Genome Editing of Rodent Intact Embryos by Electroporation , 2015, PloS one.

[21]  Jennifer A. Doudna,et al.  Generation of knock-in primary human T cells using Cas9 ribonucleoproteins , 2015, Proceedings of the National Academy of Sciences.

[22]  Israel Steinfeld,et al.  Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells , 2015, Nature Biotechnology.

[23]  T. Takemoto,et al.  Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing , 2015, Scientific Reports.

[24]  Takashi Yamamoto,et al.  Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice , 2015, Genome Biology.

[25]  Ankit Malhotra,et al.  Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease , 2015, Genetics.

[26]  Martin J. Aryee,et al.  GUIDE-Seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases , 2014, Nature Biotechnology.

[27]  Feng Zhang,et al.  Genome engineering using CRISPR-Cas9 system. , 2015, Methods in molecular biology.

[28]  B. van Steensel,et al.  Easy quantitative assessment of genome editing by sequence trace decomposition , 2014, Nucleic acids research.

[29]  Takashi Yamamoto,et al.  Simple knockout by electroporation of engineered endonucleases into intact rat embryos , 2014, Scientific Reports.

[30]  Matthew Meyerson,et al.  Targeted genomic rearrangements using CRISPR/Cas technology , 2014, Nature Communications.

[31]  Y. Arai,et al.  Validation of microinjection methods for generating knockout mice by CRISPR/Cas-mediated genome engineering , 2014, Scientific Reports.

[32]  Yoshitaka Fujihara,et al.  Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA , 2013, Scientific Reports.

[33]  James E. DiCarlo,et al.  RNA-Guided Human Genome Engineering via Cas9 , 2013, Science.

[34]  Le Cong,et al.  Multiplex Genome Engineering Using CRISPR/Cas Systems , 2013, Science.