A lignin peroxidase-encoding cDNA from the white-rot fungus Phlebia radiata: characterization and expression in Trichoderma reesei.

The nucleotide sequence of a cDNA coding for a lignin peroxidase (Lgp) of the white-rot fungus, Phlebia radiata, has been determined. By amino acid (aa) sequencing, it has been shown that the protein product of this gene is the LIII Lgp of Pb. radiata. The isolated gene and the putative aa sequence are about 60% homologous to published Lgp sequences from the fungus, Phanerochaete chrysosporium. The aa thought to be involved in the catalysis of LIII are revealed by comparison with the yeast cytochrome c peroxidase. The P. radiata Lgp-encoding gene (lgp3) was expressed in the fungus, Trichoderma reesei, under the cellobiohydrolase-encoding cbh1 gene promoter. Lgp3 mRNA was produced by the T. reesei transformants. No Lgp protein, however, could be detected.

[1]  M. Tien,et al.  Cloning and sequencing of a cDNA for a ligninase from Phanerochaete chrysosporium , 1987, Nature.

[2]  M. Niku-Paavola,et al.  Ligninolytic enzymes of the white-rot fungus Phlebia radiata. , 1988, The Biochemical journal.

[3]  M. Penttilä,et al.  EGIII, a new endoglucanase from Trichoderma reesei: the characterization of both gene and enzyme. , 1988, Gene.

[4]  T. Teeri,et al.  Construction of cDNA libraries by blunt-end ligation: high-frequency cloning of long cDNAs from filamentous fungi. , 1987, Analytical biochemistry.

[5]  R. Zagursky,et al.  Rapid and easy sequencing of large linear double-stranded DNA and supercoiled plasmid DNA , 1985 .

[6]  P. Leder,et al.  Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. , 1972, Proceedings of the National Academy of Sciences of the United States of America.

[7]  J. Garnier,et al.  Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins. , 1978, Journal of molecular biology.

[8]  J. Reiser,et al.  Molecular analysis of a Phanerochaete chrysosporium lignin peroxidase gene. , 1988, Gene.

[9]  B. Montenecourt.  Trichoderma reesei cellulases , 1983 .

[10]  K. R. Woods,et al.  Prediction of protein antigenic determinants from amino acid sequences. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[11]  R. W. Davis,et al.  Yeast RNA polymerase II genes: isolation with antibody probes. , 1983, Science.

[12]  M Penttilä,et al.  A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei. , 1987, Gene.

[13]  M. Penttilä,et al.  A Novel Fungal Expression System: Secretion of Active Calf Chymosin from the Filamentous Fungus Trichoderma Reesei , 1989, Bio/Technology.

[14]  K. Welinder,et al.  Amino-acid sequences of heme-linked, histidine-containing peptides of five peroxidases from horseradish and turnip. , 1977, European journal of biochemistry.

[15]  S. Henikoff.  Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. , 1984, Gene.

[16]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[17]  H. A. Boer,et al.  Analysis of nucleotide sequences of two ligninase cDNAs from a white-rot filamentous fungus, Phanerochaete chrysosporium. , 1987, Gene.

[18]  R. Farrell,et al.  Enzymatic "combustion": the microbial degradation of lignin. , 1987, Annual review of microbiology.

[19]  W. Rutter,et al.  Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. , 1979, Biochemistry.

[20]  M. Hynes,et al.  Isolation of genomic clones containing the amdS gene of Aspergillus nidulans and their use in the analysis of structural and regulatory mutations , 1983, Molecular and cellular biology.

[21]  G. Blobel,et al.  Nucleotide sequence of the yeast nuclear gene for cytochrome c peroxidase precursor. Functional implications of the pre sequence for protein transport into mitochondria. , 1982, The Journal of biological chemistry.

[22]  P. Broda,et al.  Multiple ligninase-related genes from Phanerochaete chrysosporium. , 1988, Gene.

[23]  B C Finzel,et al.  Crystal structure of yeast cytochrome c peroxidase refined at 1.7-A resolution. , 1984, The Journal of biological chemistry.

[24]  N. Xuong,et al.  The crystal structure of cytochrome c peroxidase. , 1980, The Journal of biological chemistry.

[25]  J. Kraut,et al.  The stereochemistry of peroxidase catalysis. , 1980, The Journal of biological chemistry.

[26]  S. Covert,et al.  Nucleotide sequence of a ligninase gene from Phanerochaete chrysosporium. , 1988, Nucleic acids research.

[27]  F. Maley,et al.  Optimizing hydrolysis of N-linked high-mannose oligosaccharides by endo-beta-N-acetylglucosaminidase H. , 1984, Analytical biochemistry.