Dietary probiotics enhance the immunity of Thai pangas (Pangasius hypophthalmus) against Pseudomonas fluorescens

The use of probiotics in aquaculture is now gaining a wide acceptance. This study was conducted to evaluate the commercially available probiotic Bioprob (PIC-BIO company, Tokyo, Japan) on the efficacy of its use on mean immune parameters and Pseudomonas fluorescens disease challenge in Thai pangas (Pangasius hypophthalmus) under laboratory conditions. The fishes were fed with five different experimental pellet diets enriched with 0 g, 0.5 g, 1 g, 1.5 g and 2 g Bioprob probiotic per kg feed that enhanced the immune potentiality investigated on weeks 1, 2 and 4. The phagocytic and bactericidal activity significantly increased in P. hypophthalmus fed with 2 g Bioprob probiotic per kg feed against P. fluorescens on weeks 2 and 4. After the feeding trial, the fishes were injected intraperiotonaly (IP) with 0.2 ml of 1.8 X 10-7 CFU ml-1 pathogenic P. fluorescens. The cumulative mortality was low in T5 (2 g kg-1) whereas high in T2 (0.5 g kg-1) as 68% compared to control (0 g kg-1) as 84% against pathogen. Therefore, dietary probiotics with 2 g kg-1 feed were found to enhance the disease resistance of P. hypophthalmus against P. fluorescens.

[1]  S. Johri,et al.  Role of Probiotics in Aquaculture , 2016 .

[2]  M. Esteban,et al.  Use of Probiotic Bacteria against Bacterial and Viral Infections in Shellfish and Fish Aquaculture , 2014 .

[3]  S. Kulabtong.  Biology and culture techniques of bocourti catfish, Pangasius bocourti Sauvage, 1880 in Thailand , 2012 .

[4]  S. S. Giri,et al.  Effects of dietary supplementation of potential probiotic Pseudomonas aeruginosa VSG-2 on the innate immunity and disease resistance of tropical freshwater fish, Labeo rohita. , 2012, Fish & shellfish immunology.

[5]  C. Lim,et al.  Use of Probiotics in Diets of Tilapia , 2011 .

[6]  Heinrich Kaspar M. Josie Lategan Lewis Gibson Aditya Kesarcodi-Watson.  Probiotics in aquaculture: The need, principles and mechanisms of action and screening processes , 2011 .

[7]  L. Olivera-Castillo,et al.  Effect of the inclusion of a bacterial mix (Streptococcus faecium and Lactobacillus acidophilus), and the yeast (Saccharomyces cerevisiae) on growth, feed utilization and intestinal enzymatic activity of Nile tilapia (Oreochromis niloticus) , 2010 .

[8]  J. Balcázar,et al.  The effect of Pediococcus acidilactici on the gut microbiota and immune status of on‐growing red tilapia (Oreochromis niloticus) , 2010, Journal of applied microbiology.

[9]  A. Shalaby,et al.  Micrococcus luteus and Pseudomonas species as probiotics for promoting the growth performance and health of Nile tilapia, Oreochromis niloticus. , 2009, Fish & shellfish immunology.

[10]  J. Jo,et al.  Use of live and dead probiotic cells in tilapia Oreochromis niloticus , 2006, Fisheries Science.

[11]  S. Aly,et al.  Studies on Bacillus subtilis and Lactobacillus acidophilus, as potential probiotics, on the immune response and resistance of Tilapia nilotica (Oreochromis niloticus) to challenge infections. , 2008, Fish & shellfish immunology.

[12]  D. Aboagye.  Evaluation of the Commercially-Available Probiotic Lymnozyme as an Effective Control of Bacterial Infections in Channel Catfish , 2008 .

[13]  P. Sherman,et al.  Lactobacillus rhamnosus Strain GG Prevents Enterohemorrhagic Escherichia coli O157:H7-Induced Changes in Epithelial Barrier Function , 2008, Infection and Immunity.

[14]  P. Menasveta,et al.  Probiotics in Aquaculture : A Case Study of Probiotics for Larvae of the Black Tiger Shrimp ( Penaeus monodon ) * 1 , 2007 .

[15]  Oncorhynchus tshawytscha.  Trials with an orally and immersion-administered P-1 , 3 glucan as an immunoprophylactic against Aeromonas salmonicida in juvenile chinook salmon Oncorhynchus tshawytscha , 2006 .

[16]  B. Austin,et al.  Use of a probiotic to control lactococcosis and streptococcosis in rainbow trout, Oncorhynchus mykiss (Walbaum). , 2005, Journal of fish diseases.

[17]  Takeshi Kobayashi,et al.  The viability of probiotic bacteria as a factor influencing the immune response in rainbow trout Oncorhynchus mykiss , 2005 .

[18]  D. Tovar‐Ramírez,et al.  Influence of dietary live yeast on European sea bass (Dicentrarchus labrax) larval development , 2004 .

[19]  B. Austin,et al.  Use of probiotics to control furunculosis in rainbow trout, Oncorhynchus mykiss (Walbaum) , 2002 .

[20]  S. Salminen,et al.  Protection of rainbow trout (Oncorhynchus mykiss) from furunculosis by Lactobacillus rhamnosus , 2001 .

[21]  L. Rahme,et al.  Common mechanisms for pathogens of plants and animals. , 2001, Annual review of phytopathology.

[22]  H. Schmidt,et al.  Potency testing of β-glucan immunostimulating effect in food for ornamental fish. , 2000 .

[23]  V. de Lorenzo,et al.  Expression systems and physiological control of promoter activity in bacteria. , 1998, Current opinion in microbiology.

[24]  J. Lamas,et al.  Efficacy of intraperitoneal and immersion vaccination against Enterococcus sp. infection in turbot , 1995 .

[25]  J. Carver.  Dietary nucleotides: cellular immune, intestinal and hepatic system effects. , 1994, The Journal of nutrition.

[26]  B. Robertsen,et al.  Adjuvant effect of a yeast glucan in vaccines against furunculosis in Atlantic salmon (Salmo salar L.) , 1993 .

[27]  K. Timmis,et al.  Redesigning metabolic routes: manipulation of TOL plasmid pathway for catabolism of alkylbenzoates. , 1987, Science.

[28]  K. N. Timmis,et al.  New Route to Bacterial Production of Indigo , 1986, Bio/Technology.

[29]  F. Rudolph,et al.  Effect of dietary nucleotides on response to bacterial infections. , 1986, JPEN. Journal of parenteral and enteral nutrition.