Online-Viabilitätsuntersuchung bei Hefezellen mit Durchfluss-Mikroskopie

various fundamental operations under the recycling strategy and substantiate GMP-compliant solutions. Design methods for process development as well as optimization strategies to improve productivity especially in product processing are shown with the help of examples. In addition, suggestions to optimize the upstream regarding the secondary components spectrum will be discussed, which will allow a cost optimized downstream processing. [1] S. Sommerfeld et al., Chem. Eng. Proc. 2005, 44, 1123. [2] J. Strube et al. in Bioseparation and Processing (Ed: G. Subramanian), Vol. 1, Wiley-VCH, Weinheim 2007, 65.