Optimization of Extraction Condition of Mycelium Polysaccharide from Tricholoma mongolium by Response Surface Methodology

In this paper, the hot water extraction conditions of mycelium polysaccharide of Tricholoma mongolium were investigated. By single-factor experiments, ethanol amount was determined at three times and the other factors (liquid–solid ratio, extraction temperature and time) were chosen to further optimize extraction conditions using response surface methodology (RSM). The Center Composite experimental results showed the optimum extraction conditions as follows: a temperature of 85.38°C, a liquid–solid ratio of 31.78 g/ mL and a extraction time of 2.18 h. Under these conditions, two extractions sufficiently reached the maximal the rate of mycelium polysaccharide 7.23%.

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