QUINONES AND α‐TOCOPHEROL IN GREENING CALLUS CULTURES OF KALANCHOË CRENATA

Summary Dark-grown Kalanchoe callus tissue subcultured into 16-hour days produce chlorophyll only after several generations. Plastoquinone: chlorophyll molar ratios in extracts from green callus indicate that the level of plastoquinone is somewhat lower than in leaf tissue. This was confirmed in purified chloroplast preparations from callus and leaves. Hill reaction activity was lower in green callus chloroplasts than in those from mature leaves. α-Tocopherol, although present in non-green callus, increased with chlorophyll synthesis. D, L[214C]-mevalonate feeding experiments seem to indicate active sites of α-tocopherol synthesis other than the mature chloroplast. Changes in ubiquinone and α-tocopherol content were observed during the growth of dark-grown callus. Such changes may be due to an increase in mitochondrial populations and to the onset of senescence.

[1]  A. Stobart,et al.  Mevalonate-activating enzymes in greening tissue cultures , 1970 .

[2]  A. Stobart,et al.  Lipids of Tissue 2Kalanchoë crenata , 1970 .

[3]  N. Weir,et al.  Phytol in tissue cultures of Kalanchoë crenata , 1969 .

[4]  A. Stobart,et al.  Chlorophyllase in tissue cultures of kalanchoë crenata , 1968 .

[5]  A. Stobart,et al.  δ-Aminolaevulinic acid dehydratase in tissue cultures of Kalanchoë crenata , 1968 .

[6]  A. Stobart,et al.  Chlorophylls and carotenoids of colourless callus, green callus and leaves of kalanchoë crenata , 1967 .

[7]  L. McLaren,et al.  CO2 FIXATION, ORGANIC ACIDS AND SOME ENZYMES IN GREEN AND COLOURLESS TISSUE CULTURES OE KALANCHOË CRENATA , 1967 .

[8]  T. Goodwin,et al.  Intracellular localization of mevalonate-activating enzymes in plant cells. , 1966, The Biochemical journal.

[9]  V. Booth.  The rise in tocopherol content in wilting and in non-illuminated leaves , 1964 .

[10]  H. Lichtenthaler.  A RAPID METHOD FOR THE IDENTIFICATION OF SMALL QUANTITIES OF LIPID-SOLUBLE VITAMINS AND QUINONES IN BIOLOGICAL MATERIAL. , 1964, Journal of chromatography.

[11]  V. Booth.  α-tocopherol, its co-occurrence with chlorophyll in chloroplasts , 1963 .

[12]  J. Friend,et al.  Studies of plastoquinone—2. Oxidation-reduction reactions of plastoquinone in isolated chloroplasts , 1963 .

[13]  F. L. Crane,et al.  Subcellular Distribution of α-Tocopherol in Spinach & Lilac Leaf Tissue , 1963 .

[14]  V. Booth.  Determination of tocopherols in plant tissues , 1963 .

[15]  V. Booth,et al.  The α‐Tocopherol content of leaves as affected by growth rate , 1961 .

[16]  F. L. Crane.  Isolation of Two Quinones with Coenzyme Q Activity from Alfalfa. , 1959, Plant physiology.

[17]  F. L. Crane,et al.  Studies on the electron transport system. XX. Chemical and physical properties of the coenzyme Q family of compounds. , 1959, Biochimica et biophysica acta.

[18]  N. Bishop.  THE REACTIVITY OF A NATURALLY OCCURRING QUINONE (Q-255) IN PHOTOCHEMICAL REACTIONS OF ISOLATED CHLOROPLASTS. , 1959, Proceedings of the National Academy of Sciences of the United States of America.

[19]  R. Lester,et al.  Chromatography of the coenzyme Q family of compounds on silicone-impregnated paper. , 1959, The Journal of biological chemistry.

[20]  V. Das,et al.  THE ORGANIZATION OF RESPIRATION IN CHLOROPHYLLOUS CELLS , 1957 .

[21]  F. Zscheile,et al.  ANALYSIS OF PLANT EXTRACTS FOR CHLOROPHYLLS a AND b BY A PHOTOELECTRIC SPECTROPHOTOMETRIC METHOD. , 1942, Plant physiology.

[22]  J. M. Vandenbelt,et al.  The ultraviolet absorption of vitamins K1, K2, and some related compounds. , 1939 .

[23]  C. Engel,et al.  Colorimetric Determination of dl-α-Tocopherol (Vitamin E) , 1938, Nature.