ZnO Nanorods Based Enzymatic Biosensor for Selective Determination of Penicillin

In this study, we have successfully demonstrated the fabrication of a biosensor based on well aligned single-crystal zinc oxide (ZnO) nanorods which were grown on gold coated glass substrate using a low temperature aqueous chemical growth (ACG) method. The ZnO nanorods were immobilized with penicillinase enzyme using the physical adsorption approach in combination with N-5-azido-2-nitrobenzoyloxysuccinimide (ANB-NOS) as cross linking molecules. The potentiometric response of the sensor configuration revealed good linearity over a large logarithmic concentration range from 100 µM to 100 mM. During the investigations, the proposed sensor showed a good stability with high sensitivity of ~121 mV/decade for sensing of penicillin. A quick electrochemical response of less than 5 s with a good selectivity, repeatability, reproducibility and a negligible response to common interferents such as Na1+, K1+, d-glucose, l-glucose, ascorbic acid, uric acid, urea, sucrose, lactose, glycine, penicilloic acid and cephalosporins, was observed.

[1]  Zhengdong Sun,et al.  Immobilization of uricase on ZnO nanorods for a reagentless uric acid biosensor , 2004 .

[2]  Andrey Bratov,et al.  Enzyme semiconductor sensor based on butyrylcholinesterase , 1991 .

[3]  Zafar Hussain Ibupoto,et al.  Selective potentiometric determination of uric acid with uricase immobilized on ZnO nanowires , 2011 .

[4]  M. Willander,et al.  Glucose Detection With a Commercial MOSFET Using a ZnO Nanowires Extended Gate , 2009, IEEE Transactions on Nanotechnology.

[5]  N. V. Shvedene,et al.  Determination of β-Lactam Antibiotics by Potentiometry with Ion-Selective Electrodes , 2003 .

[6]  B. Tay,et al.  A novel amperometric biosensor based on ZnO:Co nanoclusters for biosensing glucose. , 2007, Biosensors & bioelectronics.

[7]  H. Lüth,et al.  A long-term stable penicillin-sensitive potentiometric biosensor with enzyme immobilized by heterobifunctional cross-linking , 1996 .

[8]  Kunihiro Ichimura,et al.  Integrated enzyme fets for simultaneous detections of urea and glucose , 1985 .

[9]  P Bergveld,et al.  Highly sensitive glucose sensor based on work function changes measured by an EMOSFET. , 2003, The Analyst.

[10]  Z. Fan,et al.  Zinc oxide nanostructures: synthesis and properties. , 2005, Journal of nanoscience and nanotechnology.

[11]  Karl Schügerl,et al.  Bio-field-effect transistors for process control in biotechnology , 1991 .

[12]  Magnus Willander,et al.  A fast and sensitive potentiometric glucose microsensor based on glucose oxidase coated ZnO nanowires grown on a thin silver wire , 2010 .

[13]  M. Schöning,et al.  Recent advances in biologically sensitive field-effect transistors (BioFETs). , 2002, The Analyst.

[14]  E. Benito-Peña,et al.  Evaluation of mixed mode solid phase extraction cartridges for the preconcentration of beta-lactam antibiotics in wastewater using liquid chromatography with UV-DAD detection , 2006 .

[15]  M. Adami,et al.  Nano-assembly of glucose oxidase on the in situ self-assembled films of polypyrrole and its optical, surface and electrochemical characterizations , 2000 .

[16]  C. M. Li,et al.  Tailoring Zinc Oxide Nanowires for High Performance Amperometric Glucose Sensor , 2007 .

[17]  M. Pikkemaat,et al.  Microbial screening methods for detection of antibiotic residues in slaughter animals , 2009, Analytical and bioanalytical chemistry.

[18]  Chunzhong Li,et al.  Biosensor Based on Self‐Assembling Glucose Oxidase and Dendrimer‐Encapsulated Pt Nanoparticles on Carbon Nanotubes for Glucose Detection , 2007 .

[19]  Huai-you Wang,et al.  Simultaneous determination of carvedilol and ampicillin sodium by synchronous fluorimetry. , 2005, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy.

[20]  Karl Schügerl,et al.  Development and application of a new enzyme sensor type based on the EIS-capacitance structure for bioprocess control , 1994 .

[21]  A. A. Iozep,et al.  Spectrophotometric Determination of Penicillin Antibiotics , 2003, Pharmaceutical Chemistry Journal.

[22]  X. W. Sun,et al.  Zinc oxide nanocomb biosensor for glucose detection , 2006 .

[23]  P. Solich,et al.  Ion selective electrodes for penicillin-G based on Mn(III)TPP-Cl and their application in pharmaceutical formulations control by sequential injection analysis. , 2004, Journal of pharmaceutical and biomedical analysis.

[24]  David P. Norton,et al.  ZnO nanowire growth and devices , 2004 .

[25]  J. Janata,et al.  Field effect transistor sensitive to penicillin , 1980 .

[26]  Josep Samitier,et al.  FET‐Based Chemical Sensor Systems Fabricated with Standard Technologies , 2004 .

[27]  N. Rahman,et al.  Spectrophotometric Determination of Ampicillin, Amoxycillin, and Carbenicillin Using Folin-Ciocalteu Phenol Reagent , 2004 .

[28]  H. Hara,et al.  Analytical application for chemicals using an enzyme sensor based on an ISFET , 1993 .

[29]  Determination of Benzylpenicillin, Levomycetin (Chloramphenicol), and Tetracycline in Food Products by High-Performance Liquid Chromatography , 2001 .

[30]  B. Eggins.  Chemical Sensors and Biosensors , 2002 .

[31]  Magnus Willander,et al.  Zinc oxide nanorods grown on two-dimensional macroporous periodic structures and plane Si as a pH sensor , 2008 .

[32]  S. Enfors,et al.  Design and response characteristics of an enzyme electrode for measurement of penicillin in fermentation broth , 1979 .

[33]  E. Khamis,et al.  Use of Cerium (IV) in the Spectrophotometric and Spectrofluorimetric Determinations of Penicillins and Cephalosporins in Their Pharmaceutical Preparations , 2000 .

[34]  B. D. Malhotra,et al.  Cholesterol biosensor based on rf sputtered zinc oxide nanoporous thin film , 2007 .

[35]  Xiaoli Zhu,et al.  Electrochemical study of the effect of nano-zinc oxide on microperoxidase and its application to more sensitive hydrogen peroxide biosensor preparation. , 2007, Biosensors & bioelectronics.

[36]  E. Leszczyńska.  Biosensors with Immobilized Penicillin Amidohydrolase and Penicillinase for Determination , 1996 .