Two-photon polymerization of inorganic-organic polymers for biomedical and microoptical applications

Two-photon polymerization (2PP) is an attractive technique for the fabrication of arbitrary three-dimensional structures with feature sizes down to 100nm. In this chapter, the potential of subwavelength structures for biomedical and microoptical applications is studied. We optimized the focusing of ultrashort laser pulses and developed new materials. Specially adapted refractive-diffractive hybrid optics were designed and constructed tomaintain the sub-micrometer resolution of the fabricationprocess for the completeheight of large-scale structures.New inorganic-organic polymers were synthesized and characterized with respect to their biocompatibility and biodegradability. Additionally, molecular modeling of inorganic-organic polymers was carried out to understand the structure and dynamics of monomers and polymerization products on a molecular level. 2PP-fabricated structures for the controlled growth of human endothelia in 2D and 3D cells are presented. Finally, microlenses, diffractive optical elements, and a diaphragm array for multi-aperture camera modules were fabricated and characterized with respect to their optical performance.

[1]  H. Sun,et al.  COMPASS: An ab Initio Force-Field Optimized for Condensed-Phase ApplicationsOverview with Details on Alkane and Benzene Compounds , 1998 .

[2]  Christophe Vieu,et al.  Electron beam lithography: resolution limits and applications , 2000 .

[3]  Jan Hansmann,et al.  Vascularised human tissue models: a new approach for the refinement of biomedical research. , 2010, Journal of biotechnology.

[4]  Richard O Hynes,et al.  Integrins Bidirectional, Allosteric Signaling Machines , 2002, Cell.

[5]  Matthew Lang,et al.  Geometrical analysis of third-order aberrations for a solid immersion lens. , 2008, Optics express.

[6]  G. Zaikov.  QUANTITATIVE ASPECTS OF POLYMER DEGRADATION IN THE LIVING BODY , 1985 .

[7]  Gerhard,et al.  Two-Photon Polymerization of Inorganic-Organic Hybrid Polymers as Scalable Technology Using Ultra-Short Laser Pulses , 2010 .

[8]  M. Popall,et al.  Applications of hybrid organic–inorganic nanocomposites , 2005 .

[9]  H. Schroder,et al.  Polymer Optical Interconnects—A Scalable Large-Area Panel Processing Approach , 2006, IEEE Transactions on Advanced Packaging.

[10]  S. Steenhusen,et al.  Towards an atomistic model for ORMOCER®-I: application of forcefield methods , 2012, Journal of Sol-Gel Science and Technology.

[11]  G. Whitesides,et al.  Cell shape provides global control of focal adhesion assembly. , 2003, Biochemical and biophysical research communications.

[12]  Sophie C Cox,et al.  Biomaterial scaffolds for tissue engineering. , 2013, Frontiers in bioscience.

[13]  Adam J. Engler,et al.  Myotubes differentiate optimally on substrates with tissue-like stiffness , 2004, The Journal of cell biology.

[14]  Herbert Wolter,et al.  Synthesis, properties and applications of inorganic–organic copolymers (ORMOCER®s) , 1999 .

[15]  Martin Wegener,et al.  Tailored 3D Mechanical Metamaterials Made by Dip‐in Direct‐Laser‐Writing Optical Lithography , 2012, Advanced materials.

[16]  Elliot L Chaikof,et al.  Elastin-like protein matrix reinforced with collagen microfibers for soft tissue repair. , 2011, Biomaterials.

[17]  A Ranella,et al.  Direct laser writing of 3D scaffolds for neural tissue engineering applications , 2011, Biofabrication.

[18]  Dan Cojoc,et al.  Design and fabrication of on-fiber diffractive elements for fiber-waveguide coupling by means of e-beam lithography , 2003 .

[19]  G. Kino,et al.  Solid immersion microscope , 1990 .

[20]  Christopher S. Chen,et al.  Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment. , 2004, Developmental cell.

[21]  Andreas Tünnermann,et al.  Optical Cluster Eye fabricated on wafer-level. , 2011, Optics express.

[22]  Gary J. Swanson,et al.  Binary Optics Technology: Theoretical Limits on the Diffraction Efficiency of Multilevel Diffractive Optical Elements , 1991 .

[23]  Joyce Y. Wong,et al.  Balance of chemistry, topography, and mechanics at the cell–biomaterial interface: Issues and challenges for assessing the role of substrate mechanics on cell response , 2004 .

[24]  J. Pendry,et al.  Three-Dimensional Invisibility Cloak at Optical Wavelengths , 2010, Science.

[25]  Georg Jakopic,et al.  Two-photon 3D lithography: A Versatile Fabrication Method for Com- plex 3D Shapes and Optical Interconnects within the Scope of Innovative Industrial Applications , 2007 .

[26]  Saulius Juodkazis,et al.  Photopolymerized microscopic vortex beam generators: Precise delivery of optical orbital angular momentum , 2010 .

[27]  M. Wegener,et al.  Two‐Component Polymer Scaffolds for Controlled Three‐Dimensional Cell Culture , 2011, Advanced materials.

[28]  Alexander Jesacher,et al.  Three dimensional laser microfabrication in diamond using a dual adaptive optics system. , 2011, Optics express.

[29]  Andreas Tünnermann,et al.  Materials and technologies for fabrication of three-dimensional microstructures with sub-100 nm feature sizes by two-photon polymerization , 2012 .

[30]  Joyce Y Wong,et al.  Direct comparison of the spread area, contractility, and migration of balb/c 3T3 fibroblasts adhered to fibronectin- and RGD-modified substrata. , 2004, Biophysical journal.

[31]  L G Griffith,et al.  Cell adhesion and motility depend on nanoscale RGD clustering. , 2000, Journal of cell science.

[32]  Erich Wintermantel,et al.  Medizintechnik Life Science Engineering , 2008 .

[33]  C. S. Chen,et al.  Geometric control of cell life and death. , 1997, Science.

[34]  Francesco Gentile,et al.  Optical micro-structures fabricated on top of optical fibers by means of two-photon photopolymerization , 2010 .

[35]  B. Chichkov,et al.  Two photon induced polymerization of organic-inorganic hybrid biomaterials for microstructured medical devices. , 2006, Acta biomaterialia.

[36]  J W Melvin,et al.  Failure properties of passive human aortic tissue. II--Biaxial tension tests. , 1983, Journal of biomechanics.

[37]  Elliot L Chaikof,et al.  Acellular vascular grafts generated from collagen and elastin analogs. , 2013, Acta biomaterialia.

[38]  S. Kawata,et al.  Three-dimensional microfabrication with two-photon-absorbed photopolymerization. , 1997, Optics letters.

[39]  R. Gadonas,et al.  Femtosecond laser polymerization of hybrid/integrated micro-optical elements and their characterization , 2010 .

[40]  K E Healy,et al.  Kinetics of bone cell organization and mineralization on materials with patterned surface chemistry. , 1996, Biomaterials.

[41]  Hiroaki Misawa,et al.  Three-dimensional photonic crystal structures achieved with two-photon-absorption photopolymerization of resin , 1999 .

[42]  Koichi Masuda,et al.  Tensile mechanical properties of bovine articular cartilage: Variations with growth and relationships to collagen network components , 2003, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[43]  Benjamin G Keselowsky,et al.  Surface chemistry modulates fibronectin conformation and directs integrin binding and specificity to control cell adhesion. , 2003, Journal of biomedical materials research. Part A.

[44]  Boris N. Chichkov,et al.  Biocompatibility of CAD/CAM ORMOCER polymer scaffold structures , 2004 .

[45]  L. Addadi,et al.  Hierarchical assembly of cell-matrix adhesion complexes. , 2004, Biochemical Society transactions.

[46]  Jang‐Ung Park,et al.  Inorganic–organic hybrid materials for application in optical devices , 2002 .

[47]  A. Tünnermann,et al.  High numerical aperture hybrid optics for two-photon polymerization. , 2012, Optics express.

[48]  P. Dannberg,et al.  ORMOCER®s for Optical Interconnection Technology , 2001 .

[49]  Min Gu,et al.  Direct optical fabrication of three-dimensional photonic crystals in a high refractive index LiNbO3 crystal. , 2006, Optics letters.

[50]  Bennett B. Goldberg,et al.  Theoretical analysis of numerical aperture increasing lens microscopy , 2005 .

[51]  Mangirdas Malinauskas,et al.  In vitro and in vivo biocompatibility study on laser 3D microstructurable polymers , 2012 .

[52]  J W Melvin,et al.  Failure properties of passive human aortic tissue. I--uniaxial tension tests. , 1982, Journal of biomechanics.

[53]  Hong-Bo Sun,et al.  High numerical aperture microlens arrays of close packing , 2010 .

[54]  T F Lue,et al.  Composition and biomechanical properties of the bladder acellular matrix graft: comparative analysis in rat, pig and human. , 1998, British journal of urology.

[55]  Jesper Serbin,et al.  Advanced packaging materials for optical applications: bridging the gap between nm-size structures and large-area panel processing , 2006, SPIE OPTO.

[56]  Markus Pessa,et al.  Nanoimprint Lithography - Next Generation Nanopatterning Methods for Nanophotonics Fabrication , 2010 .

[57]  Andreas Tünnermann,et al.  Thin wafer-level camera lenses inspired by insect compound eyes. , 2010, Optics express.

[58]  Robert Leitel,et al.  Multi-aperture optics for wafer-level cameras , 2011 .

[59]  Andreas Tünnermann,et al.  Hybrid optics for focusing ultrashort laser pulses. , 2006, Optics letters.

[60]  Min Gu,et al.  Near-infrared photonic crystals with higher-order bandgaps generated by two-photon photopolymerization. , 2002, Optics letters.

[61]  Byoungho Lee,et al.  Use of two-photon polymerization for continuous gray-level encoding of diffractive optical elements , 2007 .

[62]  B N Chichkov,et al.  Femtosecond laser-induced two-photon polymerization of inorganic-organic hybrid materials for applications in photonics. , 2003, Optics letters.