Differential diagnosis of Entamoeba spp. in gastrointestinal disorder patients in Khorramabad, Iran

Differential diagnosis of Entamoeba spp. has great clinical and epidemiological importance. Entamoeba moshkovskii cysts and trophozoites are morphologically indistinguishable fromEntamoeba dispar and Entamoeba histolytica. This study was carried out for the first time to detect Entamoeba spp. in stool samples by using molecular method from April 2010 to December 2010 in Khorramabad, Iran. A total of 862 fecal specimens were collected from patients having abnormal gastrointestinal symptoms and who were referred to the health care centers of Khorramabad. Out of 862 stool samples, 16 (1.86 %) showed the presence of E. histolytica/E. dispar/E. moshkovskii cysts by microscopic examination. Consequently, single-round PCR was carried out to differentiate the Entamoeba spp. OF the sixteen samples that were microscopically positive, 1 (6.25%) was E. moshkovskii, and 15 (93.75%) were E. dispar. Our results, along with those of other similar study conducted in different parts of Iran, reveal that E. dispar is more prevalent.   Key words: Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, single-round PCR, Iran.

[1]  B. Kazemi,et al.  Discrimination of Entamoeba moshkovskii in patients with gastrointestinal disorders by single-round PCR. , 2010, Japanese journal of infectious diseases.

[2]  Navid Sahebekhtiari,et al.  Characterization of Entamoeba histolytica and Entamoeba dispar in fresh stool by PCR. , 2009 .

[3]  E. Houpt,et al.  High prevalence of Entamoeba moshkovskii in a Tanzanian HIV population. , 2008, Acta tropica.

[4]  D. Stark,et al.  Entamoeba moshkovskii infections in Sydney, Australia , 2008, European Journal of Clinical Microbiology & Infectious Diseases.

[5]  D. Marriott,et al.  Laboratory Diagnostic Techniques for Entamoeba Species , 2007, Clinical Microbiology Reviews.

[6]  M. Azimirad,et al.  Prevalence of Entamoeba histolytica and Entamoeba dispar in Gonbad City, 2006, Iran , 2007 .

[7]  O. Koru,et al.  Two cases of rarely recognized infection with Entamoeba moshkovskii. , 2007, The American journal of tropical medicine and hygiene.

[8]  N. Beebe,et al.  PCR Detection of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii in Stool Samples from Sydney, Australia , 2007, Journal of Clinical Microbiology.

[9]  P. Chavalitshewinkoon-Petmitr,et al.  Differential Detection of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii by a Single-Round PCR Assay , 2006, Journal of Clinical Microbiology.

[10]  W. Petri,et al.  Comparison of a Stool Antigen Detection Kit and PCR for Diagnosis of Entamoeba histolytica and Entamoeba dispar Infections in Asymptomatic Cyst Passers in Iran , 2006, Journal of Clinical Microbiology.

[11]  Krishna Khairnar,et al.  Entamoeba moshkovskii and Entamoeba dispar-associated infections in pondicherry, India. , 2005, Journal of health, population, and nutrition.

[12]  B. Kazemi,et al.  The distribution of Entamoeba histolytica and Entamoeba dispar in northern, central, and southern Iran , 2004, Parasitology Research.

[13]  R. Haque,et al.  Entamoeba moshkovskii Infections in Children in Bangladesh , 2003, Emerging infectious diseases.

[14]  C. Lengeler,et al.  Species-specific field testing of Entamoeba spp. in an area of high endemicity. , 2002, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[15]  Z. Bisoffi,et al.  Amebic infections due to the Entamoeba histolytica-Entamoeba dispar complex: a study of the incidence in a remote rural area of Ecuador. , 2002, The American journal of tropical medicine and hygiene.

[16]  R. Vines,et al.  Estimating the impact of amebiasis on health. , 2000, Parasitology today.

[17]  Seiki Kobayashi,et al.  Asymptomatic cyst passers of Entamoeba histolytica but not Entamoeba dispar in institutions for the mentally retarded in Japan. , 2000, Parasitology international.

[18]  E. Tannich.  Royal Society of Tropical Medicine and Hygiene Meeting at Manson House, London, 19 February 1998. Amoebic disease. Entamoeba histolytica and E. dispar: comparison of molecules considered important for host tissue destruction. , 1998, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[19]  C. Clark.  Entamoeba dispar, an organism reborn , 1998 .

[20]  R. Haque,et al.  Comparison of PCR, Isoenzyme Analysis, and Antigen Detection for Diagnosis of Entamoeba histolyticaInfection , 1998, Journal of Clinical Microbiology.

[21]  C. Clark.  Royal Society of Tropical Medicine and Hygiene meeting at Manson House, London, 19 February 1998. Amoebic disease. Entamoeba dispar, an organism reborn. , 1998, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[22]  L. S. Diamond,et al.  Intraspecific Variation and Phylogenetic Relationships in the Genus Entamoeba as Revealed by Riboprinting , 1997, The Journal of eukaryotic microbiology.

[23]  Entamoeba taxonomy. , 1997, Bulletin of the World Health Organization.

[24]  C. Clark,et al.  A Redescription of Entamoeba Histolytica Schaudinn, 1903 (Emended Walker, 1911) Separating It From Entamoeba Dispar Brumpt, 1925 1 , 1993, The Journal of eukaryotic microbiology.

[25]  C. Clark,et al.  The Laredo strain and other 'Entamoeba histolytica-like' amoebae are Entamoeba moshkovskii. , 1991, Molecular and biochemical parasitology.

[26]  A. Dávalos.  [LABORATORY DIAGNOSIS OF AMEBIASIS]. , 1964, Cirugia y cirujanos.