SEROLOGIC DEMONSTRATION OF DUAL S P E C I F I C I T Y OF RABBIT BIVALENT H Y B R I D ANTIBODY* BY H. H. FUDENBERG, M.D., GENEVIEVE DREWS, M.D., AWl)

Treatment of rabbit 7S antibody with pepsin results in the removal of an inactive fragment, leaving a bivalent residue with an average molecular weight of 106,000 and a sedimentation coefficient of approximately 5S (1, 2). Subsequent reduction of one labile disulfide bond splits this residual molecule into two univalent, non-precipitating fragments which migrate as a single 3.5S peak (3) in the ultracentrifuge. These fragments closely resemble the univalent fractions of a papain digest (4) in a number of their properties.

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