EFFECTS OF UV, SUNLIGHT AND X‐RAY RADIATION ON QUIESCENT HUMAN CELLS IN CULTURE

Nondividing human diploid fibroblasts (HDF) in culture have been used to study the effect on cell lethality of ultraviolet light, natural sunlight and X‐rays. A lethal effect is defined as cellular degeneration, loss from the culture and inability to exclude vital strains. Far‐and mid‐UV have a readily observable lethal effect (cell loss), with DNA and DNA damage as the critical target and critical damage respectively. In part, natural sunlight kills cells by a similar mechanism but has an additional lethal effect at longer exposure times. This additional effect is expressed by the retention of the dead cells in culture, in contrast to the UV‐induced promotion of cell degeneration and loss. Relatively large doses of X‐rays that destroy proliferative capacity, have no detectable lethal effect on the maintenance of nondividing cells. The biological response of nondividing HDF to radiations from different parts of the electromagnetic spectrum is dissimilar.

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