Accurate and reproducible detection of proteins in water using an extended-gate type organic transistor biosensor

In this Letter, we describe an accurate antibody detection method using a fabricated extended-gate type organic field-effect-transistor (OFET), which can be operated at below 3 V. The protein-sensing portion of the designed device is the gate electrode functionalized with streptavidin. Streptavidin possesses high molecular recognition ability for biotin, which specifically allows for the detection of biotinylated proteins. Here, we attempted to detect biotinylated immunoglobulin G (IgG) and observed a shift of threshold voltage of the OFET upon the addition of the antibody in an aqueous solution with a competing bovine serum albumin interferent. The detection limit for the biotinylated IgG was 8 nM, which indicates the potential utility of the designed device in healthcare applications.

[1]  P. Holt,et al.  An IgG subclass imbalance in connective tissue disease. , 1988, Annals of the rheumatic diseases.

[2]  Takao Someya,et al.  Effects of the alkyl chain length in phosphonic acid self-assembled monolayer gate dielectrics on the performance and stability of low-voltage organic thin-film transistors , 2009 .

[3]  Wolfgang B Fischer,et al.  Ultrasensitive in situ label-free DNA detection using a GaN nanowire-based extended-gate field-effect-transistor sensor. , 2011, Analytical chemistry.

[4]  B. de Boer,et al.  Tuning of Metal Work Functions with Self‐Assembled Monolayers , 2005 .

[5]  Feng Yan,et al.  Organic Thin‐Film Transistors for Chemical and Biological Sensing , 2012, Advanced materials.

[6]  Maria Magliulo,et al.  An analytical model for bio-electronic organic field-effect transistor sensors , 2013 .

[7]  Wolfgang Knoll,et al.  In situ antibody detection and charge discrimination using aqueous stable pentacene transistor biosensors. , 2011, Journal of the American Chemical Society.

[8]  Kyriaki Manoli,et al.  Organic field-effect transistor sensors: a tutorial review. , 2013, Chemical Society reviews.

[9]  C. Rossi,et al.  Quartz crystal microbalance immunosensor for the quantification of immunoglobulin G in bovine milk. , 2013, Biosensors & bioelectronics.

[10]  M Facchini,et al.  DNA adsorption measured with ultra-thin film organic field effect transistors. , 2009, Biosensors & bioelectronics.

[11]  T. Lybrand,et al.  Streptavidin-biotin binding energetics. , 1999, Biomolecular engineering.

[12]  D. Kumaki,et al.  Surface-energy-dependent field-effect mobilities up to 1 cm2/V s for polymer thin-film transistor , 2009 .

[13]  H. Klauk,et al.  Ultralow-power organic complementary circuits , 2007, Nature.

[14]  Maxim Shkunov,et al.  Liquid-crystalline semiconducting polymers with high charge-carrier mobility , 2006, Nature materials.

[15]  D. Kumaki,et al.  Influence of H2O and O2 on threshold voltage shift in organic thin-film transistors: Deprotonation of SiOH on SiO2 gate-insulator surface , 2008 .

[16]  A. Chapman-Smith,et al.  Molecular biology of biotin attachment to proteins. , 1999, The Journal of nutrition.

[17]  R. Carbonell,et al.  Dynamic and equilibrium performance of sensors based on short peptide ligands for affinity adsorption of human IgG using surface plasmon resonance. , 2014, Biosensors & bioelectronics.

[18]  Shaoyi Jiang,et al.  Probing the orientation of surface-immobilized immunoglobulin G by time-of-flight secondary ion mass spectrometry. , 2004, Langmuir : the ACS journal of surfaces and colloids.

[19]  Jang‐Joo Kim,et al.  Effect of passivation on the sensitivity and stability of pentacene transistor sensors in aqueous media. , 2011, Biosensors & bioelectronics.

[20]  Lakshminarayan K. Jagannathan,et al.  Label-free low-cost disposable DNA hybridization detection systems using organic TFTs , 2007, 2007 IEEE International Electron Devices Meeting.

[21]  R. Pei,et al.  Amplification of antigen-antibody interactions based on biotin labeled protein-streptavidin network complex using impedance spectroscopy. , 2001, Biosensors & bioelectronics.