Fluorometric measurement of aqueous ammonium ion in a flow injection system

An automated flow-injection method for NH/sub 3//NH/sub 4//sup +/ involving the ternary reaction of the analyte with o-phthaldialdehyde (OPA) and sulfite is described. The use of malodorous thiol compounds is avoided and the reaction provides much greater sensitivity by either fluorescence (detection limit 300 fmol or 20 nM NH/sub 4//sup +/) or absorption detection compared to the reaction involving 2-mercaptoethanol. The reaction shows considerable selectivity for ammonia over amino acids by a factor of 16 to > 500 for 11 common amino acids studied. The throughput rate is 25 samples/h.

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