Profile of the skin microbiota in a healthy Chinese population

The skin microbiota is an inseparable component of the skin barrier structure, which participates in the stabilization or impairment of the barrier function as well as the development of many skin diseases. To characterize the normal skin microbiota and its association with skin sites, age and sex, we recruited 50 volunteers divided into children, adolescents, young adults, middle‐aged adults and the elderly. The skin sites consisted of cheeks, volar forearms (representing dry environments) and upper back (representing sebaceous environments). A total of 9 574 365 high‐quality sequences of the V3 to V4 region of the 16S rRNA gene were annotated with taxonomic information related to two archaeal phyla (Thaumarchaeota and Euryarchaeota) and five dominant bacterial phyla (Actinobacteria, Proteobacteria, Firmicutes, Bacteroidetes and Cyanobacteria). The skin bacteria community structure was influenced by skin sites, and was closely related to age and sex. The upper back was dominated by Propionibacterium and Staphylococcus, and the cheeks facilitated the survival of Betaproteobacteria, while Alphaproteobacteria were prevalent on the volar forearms. Regarding the effects of age, after sexual maturity, the cheek microbiota became more similar to sebaceous sites (i.e. the upper back). The volar forearms appeared to experience the aging process earlier than the other two sites. The elderly had greater species richness and diversity and their community composition no longer had skin‐site selectivity. Males had a greater species richness than females, but the sex differences in the community structure only present at certain age groups and skin sites.

[1]  Alexander J. Probst,et al.  Human age and skin physiology shape diversity and abundance of Archaea on skin , 2017, Scientific Reports.

[2]  Martin J. Blaser,et al.  Body Site Is a More Determinant Factor than Human Population Diversity in the Healthy Skin Microbiome , 2016, PloS one.

[3]  Patrick K. H. Lee,et al.  Erratum: Insights into the pan-microbiome: skin microbial communities of Chinese individuals differ from other racial groups , 2016, Scientific Reports.

[4]  J. Gilbert,et al.  The Influence of Age and Gender on Skin-Associated Microbial Communities in Urban and Rural Human Populations , 2015, PloS one.

[5]  Patrick K. H. Lee,et al.  Insights into the pan-microbiome: skin microbial communities of Chinese individuals differ from other racial groups , 2015, Scientific Reports.

[6]  Yang Yu,et al.  The role of the cutaneous microbiome in skin cancer: lessons learned from the gut. , 2015, Journal of drugs in dermatology : JDD.

[7]  R. Schwabe,et al.  The Microbiome and Cancer , 2021, Gut Feelings.

[8]  Alexander J. Probst,et al.  Archaea on Human Skin , 2013, PloS one.

[9]  Julia Oh,et al.  Shifts in human skin and nares microbiota of healthy children and adults , 2012, Genome Medicine.

[10]  S. Sørensen,et al.  Selection for Cu-Tolerant Bacterial Communities with Altered Composition, but Unaltered Richness, via Long-Term Cu Exposure , 2012, Applied and Environmental Microbiology.

[11]  Julia Oh,et al.  Temporal shifts in the skin microbiome associated with disease flares and treatment in children with atopic dermatitis , 2012, Genome research.

[12]  M. Boguniewicz,et al.  Atopic dermatitis: a disease of altered skin barrier and immune dysregulation , 2011, Immunological reviews.

[13]  D. Bliss,et al.  Dry skin in older adults. , 2011, Geriatric nursing.

[14]  S. Dowd,et al.  Diversity of the Human Skin Microbiome Early in Life , 2011, The Journal of investigative dermatology.

[15]  R. Gallo,et al.  Microbial Symbiosis with the Innate Immune Defense System of the Skin , 2011, The Journal of investigative dermatology.

[16]  T. Staudinger,et al.  Molecular analysis of the prevalent microbiota of human male and female forehead skin compared to forearm skin and the influence of make‐up , 2011, Journal of applied microbiology.

[17]  R. Knight,et al.  Moving pictures of the human microbiome , 2011, Genome Biology.

[18]  B. Dridi,et al.  Archaea as emerging organisms in complex human microbiomes. , 2011, Anaerobe.

[19]  T. Wolfsberg,et al.  Matriptase-deficient mice exhibit ichthyotic skin with a selective shift in skin microbiota. , 2009, The Journal of investigative dermatology.

[20]  P. Elias,et al.  Variation of Skin Surface pH, Sebum Content and Stratum Corneum Hydration with Age and Gender in a Large Chinese Population , 2009, Skin Pharmacology and Physiology.

[21]  C. Deming,et al.  Topographical and Temporal Diversity of the Human Skin Microbiome , 2009, Science.

[22]  J. Brandner,et al.  The skin: an indispensable barrier , 2008, Experimental dermatology.

[23]  R. Knight,et al.  The influence of sex, handedness, and washing on the diversity of hand surface bacteria , 2008, Proceedings of the National Academy of Sciences.

[24]  R. Wilson,et al.  Genomic and metabolic adaptations of Methanobrevibacter smithii to the human gut , 2007, Proceedings of the National Academy of Sciences.

[25]  Rajul A. Patel,et al.  Evaluation of gender difference in skin type and pH. , 2006, Journal of dermatological science.

[26]  C. Zouboulis,et al.  Chronological ageing and photoageing of the human sebaceous gland , 2001, Clinical and experimental dermatology.

[27]  J. Peloso,et al.  Evaluation of skin bacterial flora before and after aseptic preparation of clipped and nonclipped arthrocentesis sites in horses. , 1997, Veterinary surgery : VS.

[28]  J. Leyden,et al.  Age-related changes in the resident bacterial flora of the human face. , 1975, The Journal of investigative dermatology.

[29]  M. McBride,et al.  An evaluation of techniques for sampling skin flora. , 1970, The Journal of investigative dermatology.

[30]  J. Schjoerring,et al.  Bacterial diversity in Greenlandic soils as affected by potato cropping and inorganic versus organic fertilization , 2013, Polar Biology.

[31]  S. Lahtinen,et al.  The Potential of Probiotics and Prebiotics for Skin Health , 2010 .

[32]  G. Webster.  Skin microecology: the old and the new. , 2007, Archives of dermatology.