Translational readthrough of an amber termination codon during synthesis of feline leukemia virus protease

Feline leukemia virus contains a protease which apparently has the same specificity as murine leukemia virus protease. It cleaves in vitro the Pr65gag of Gazdar-mouse sarcoma virus into the constituent p15, p12, p30, and p10 proteins. We purified the protease and determined its NH2-terminal amino acid sequence (the first 15 residues). Alignment of this amino acid sequence with the nucleotide sequence (I. Laprevotte, A. Hampe, C. H. Sherr, and F. Galibert, J. Virol. 50:884-894, 1984) reveals that the protease is a viral-coded enzyme and is located at the 5' end of the pol gene. As previously found for murine leukemia virus (Y. Yoshinaka, I. Katoh, T. D. Copeland, and S. Oroszlan, Proc. Natl. Acad. Sci. U.S.A. 82:1618-1622, 1985), feline leukemia virus protease is synthesized through in-frame suppression of the gag amber termination codon by insertion of a glutamine in the fifth position, and the first four amino acids are derived from the gag gene.

[1]  I. Katoh,et al.  Murine leukemia virus protease is encoded by the gag-pol gene and is synthesized through suppression of an amber termination codon. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[2]  W. Hardy.  Feline Leukemia and Sarcoma Viruses , 1985 .

[3]  T. Copeland,et al.  Primary structure and processing of gag and env gene products of human T-cell leukemia viruses HTLV-ICR and HTLV-IATK. , 1985, Current topics in microbiology and immunology.

[4]  F. Galibert,et al.  Nucleotide sequence of the gag gene and gag-pol junction of feline leukemia virus , 1984, Journal of virology.

[5]  I. Verma,et al.  Two base changes restore infectivity to a noninfectious molecular clone of Moloney murine leukemia virus (pMLV-1) , 1984, Journal of virology.

[6]  J. F. Atkins,et al.  Resolution of the discrepancy between a gene translation--termination codon and the deduced sequence for release of the encoded polypeptide. , 1983, European journal of biochemistry.

[7]  D. Hatfield,et al.  Characterization and nucleotide sequence of a chicken gene encoding an opal suppressor tRNA and its flanking DNA segments. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[8]  M. Morgan,et al.  Structural and antigenic analysis of the nucleic acid-binding proteins of bovine and feline leukemia viruses , 1983, Journal of virology.

[9]  J. Miller,et al.  Effects of surrounding sequence on the suppression of nonsense codons. , 1983, Journal of molecular biology.

[10]  Y. Yoshinaka,et al.  p65 of Gazdar murine sarcoma viruses contains antigenic determinants from all four of the murine leukemia virus (MuLV) gag polypeptides (p15, p12, p30, and p10) and can be cleaved in vitro by the MuLV proteolytic activity. , 1982, Virology.

[11]  L. Hood,et al.  A gas-liquid solid phase peptide and protein sequenator. , 1981, The Journal of biological chemistry.

[12]  T. Fox,et al.  Leaky +1 and −1 frameshift mutations at the same site in a yeast mitochondrial gene , 1980, Nature.

[13]  T. Copeland,et al.  Separation of amino acid phenylthiohydantoins by high-performance liquid chromatography on phenylalkyl support. , 1980, Analytical biochemistry.

[14]  R. Gilden,et al.  Chapter 8 – Primary Structure Analysis of Retrovirus Proteins , 1980 .

[15]  Y. Yoshinaka,et al.  Properties of a P70 proteolytic factor of murine leukemia viruses , 1977, Cell.

[16]  A. Gazdar,et al.  Presence of sarcoma genome in a "non-infectious" mammalian virus. , 1971, Nature: New biology.

[17]  C. Rickard,et al.  A transmissible virus-induced lymphocytic leukemia of the cat. , 1969, Journal of the National Cancer Institute.

[18]  K. Habel.  Tumor viruses. , 1965, The Yale journal of biology and medicine.