Dose-dependent osteogenic effect of octacalcium phosphate on mouse bone marrow stromal cells.

Octacalcium phosphate (OCP) has been advocated to be a precursor of biological apatite crystals in bones and teeth. Our previous studies showed that synthetic OCP stimulates bone regeneration, followed by the progressive conversion of OCP into hydroxyapatite (HA), when implanted in bone defects. However, the precise mechanism to induce the osteogenic phenotype in osteoblasts by OCP has not been identified. The present study was designed to investigate whether the physicochemical aspect, specific to and derived from the structural properties of OCP, influences the function of an osteoblastic cell line, mouse bone marrow stromal ST-2 cells. Different amounts of synthetic OCP and synthetic sintered ceramic HA were coated onto 48-well tissue culture plates. The amounts of OCP and HA were controlled to strengthen their intrinsic physicochemical properties, in which the milieu around the crystals will be modified during the culture. The roughness of the OCP coatings was independent of the amount of coating. Che...

[1]  T. Martin,et al.  Origin of osteoclasts: mature monocytes and macrophages are capable of differentiating into osteoclasts under a suitable microenvironment prepared by bone marrow-derived stromal cells. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[2]  W. E. Brown,et al.  The effects of magnesium and fluoride on the hydrolysis of octacalcium phosphate. , 1992, Archives of oral biology.

[3]  M. Takeichi,et al.  Functional correlation between cell adhesive properties and some cell surface proteins , 1977, The Journal of cell biology.

[4]  G. Beck,et al.  Osteopontin Regulation by Inorganic Phosphate Is ERK1/2-, Protein Kinase C-, and Proteasome-dependent* , 2003, Journal of Biological Chemistry.

[5]  J. Voegel,et al.  First experimental evidence for human dentine crystal formation involving conversion of octacalcium phosphate to hydroxyapatite. , 1998, Acta crystallographica. Section D, Biological crystallography.

[6]  O. Suzuki,et al.  Bone formation on synthetic precursors of hydroxyapatite. , 1991, The Tohoku journal of experimental medicine.

[7]  Nicole J. Crane,et al.  Raman spectroscopic evidence for octacalcium phosphate and other transient mineral species deposited during intramembranous mineralization. , 2006, Bone.

[8]  G. Karsenty,et al.  Osf2/Cbfa1: A Transcriptional Activator of Osteoblast Differentiation , 1997, Cell.

[9]  R. Chiesa,et al.  Comparative in vitro study on a ultra-high roughness and dense titanium coating. , 2005, Biomaterials.

[10]  G S Stein,et al.  Molecular mechanisms mediating proliferation/differentiation interrelationships during progressive development of the osteoblast phenotype. , 1993, Endocrine reviews.

[11]  E Ruoslahti,et al.  New perspectives in cell adhesion: RGD and integrins. , 1987, Science.

[12]  G. Stein,et al.  The influence of type I collagen on the development and maintenance of the osteoblast phenotype in primary and passaged rat calvarial osteoblasts: modification of expression of genes supporting cell growth, adhesion, and extracellular matrix mineralization. , 1995, Experimental cell research.

[13]  K. Hitomi,et al.  DEMONSTRATION OF ALKALINE PHOSPHATASE PARTICIPATION IN THE MINERALIZATION OF OSTEOBLASTS BY ANTISENSE RNA APPROACH , 1996, Cell biology international.

[14]  A. W. Frazier,et al.  Octacalcium Phosphate and Hydroxyapatite: Crystallographic and Chemical Relations between Octacalcium Phosphate and Hydroxyapatite , 1962, Nature.

[15]  L. Rimondini,et al.  In Vitro Behaviour of Osteoblasts Cultured on Orthopaedic Biomaterials with Different Surface Roughness, Uncoated and Fluorohydroxyapatite-Coated, Relative to the in Vivo Osteointegration Rate , 2003, The International journal of artificial organs.

[16]  J. Polak,et al.  Use of green fluorescent fusion protein to track activation of the transcription factor osterix during early osteoblast differentiation. , 2005, Biochemical and biophysical research communications.

[17]  M. McKee,et al.  Unique coexpression in osteoblasts of broadly expressed genes accounts for the spatial restriction of ECM mineralization to bone. , 2005, Genes & development.

[18]  D. Deligianni,et al.  Effect of surface roughness of hydroxyapatite on human bone marrow cell adhesion, proliferation, differentiation and detachment strength. , 2001, Biomaterials.

[19]  T. Kokubo,et al.  Bioactive glass ceramics: properties and applications. , 1991, Biomaterials.

[20]  J. Deng,et al.  The Novel Zinc Finger-Containing Transcription Factor Osterix Is Required for Osteoblast Differentiation and Bone Formation , 2002, Cell.

[21]  O. Suzuki,et al.  Comparative Study on Osteoconductivity by Synthetic Octacalcium Phosphate and Sintered Hydroxyapatite in Rabbit Bone Marrow , 2006, Calcified Tissue International.

[22]  O. Suzuki,et al.  Maclura pomifera agglutinin-binding glycoconjugates on converted apatite from synthetic octacalcium phosphate implanted into subperiosteal region of mouse calvaria. , 1993, Bone and mineral.

[23]  O. Suzuki,et al.  Implanted octacalcium phosphate is more resorbable than beta-tricalcium phosphate and hydroxyapatite. , 2002, Journal of biomedical materials research.

[24]  J. Ko,et al.  Effect of electrostatic interaction on the adsorption of globular proteins on octacalcium phosphate crystal film. , 2002, Journal of colloid and interface science.

[25]  H. Ohgushi,et al.  Osteoblastic phenotype expression on the surface of hydroxyapatite ceramics. , 1997, Journal of biomedical materials research.

[26]  R. Shelton,et al.  Bone marrow cell gene expression and tissue construct assembly using octacalcium phosphate microscaffolds. , 2006, Biomaterials.

[27]  D. Ward,et al.  Physiological changes in extracellular calcium concentration directly control osteoblast function in the absence of calciotropic hormones. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[28]  Makoto Sato,et al.  Targeted Disruption of Cbfa1 Results in a Complete Lack of Bone Formation owing to Maturational Arrest of Osteoblasts , 1997, Cell.

[29]  R. Shelton,et al.  Influence of calcium phosphate crystal assemblies on the proliferation and osteogenic gene expression of rat bone marrow stromal cells. , 2007, Biomaterials.

[30]  E. Moran,et al.  Phosphate is a specific signal for induction of osteopontin gene expression. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[31]  R. Legeros,et al.  Preparation of octacalcium phosphate (OCP): A direct fast method , 1985, Calcified Tissue International.

[32]  I. Mihailescu,et al.  Human osteoblast response to pulsed laser deposited calcium phosphate coatings. , 2005, Biomaterials.

[33]  S. Heo,et al.  Microrough titanium surface affects biologic response in MG63 osteoblast-like cells. , 2006, Journal of biomedical materials research. Part A.

[34]  Y. Yamaguchi,et al.  High-resolution electron microscopical observations of initial enamel crystals , 1997 .

[35]  O. Suzuki,et al.  Reversible Structural Changes of Octacalcium Phosphate and Labile Acid Phosphate , 1995, Journal of dental research.

[36]  C. Adams,et al.  Inorganic phosphate induces apoptosis of osteoblast-like cells in culture. , 2000, Bone.

[37]  M. Grynpas,et al.  Transient precursor strategy or very small biological apatite crystals? , 2007, Bone.

[38]  C. V. van Blitterswijk,et al.  Osteogenecity of octacalcium phosphate coatings applied on porous metal implants. , 2003, Journal of biomedical materials research. Part A.

[39]  O. Suzuki,et al.  Surface chemistry and biological responses to synthetic octacalcium phosphate. , 2006, Journal of biomedical materials research. Part B, Applied biomaterials.

[40]  Clemens A van Blitterswijk,et al.  Biological performance of uncoated and octacalcium phosphate-coated Ti6Al4V. , 2005, Biomaterials.

[41]  C. Mou,et al.  Solid-state NMR study of the transformation of octacalcium phosphate to hydroxyapatite: a mechanistic model for central dark line formation. , 2006, Journal of the American Chemical Society.

[42]  R. Kamijo,et al.  Bone formation enhanced by implanted octacalcium phosphate involving conversion into Ca-deficient hydroxyapatite. , 2006, Biomaterials.

[43]  J. Aubin,et al.  Inorganic phosphate added exogenously or released from beta-glycerophosphate initiates mineralization of osteoid nodules in vitro. , 1992, Bone and mineral.

[44]  B D Boyan,et al.  Effect of titanium surface roughness on proliferation, differentiation, and protein synthesis of human osteoblast-like cells (MG63). , 1995, Journal of biomedical materials research.

[45]  C A van Blitterswijk,et al.  In vitro and in vivo degradation of biomimetic octacalcium phosphate and carbonate apatite coatings on titanium implants. , 2003, Journal of biomedical materials research. Part A.

[46]  O. Suzuki,et al.  New scaffold for recombinant human bone morphogenetic protein-2. , 2004, Journal of biomedical materials research. Part A.

[47]  T. Aoba,et al.  Preparation of Hydroxyapatite Crystals and Their Behavior as Seeds for Crystal Growth , 1984, Journal of dental research.

[48]  C. V. van Blitterswijk,et al.  Bone tissue engineering on amorphous carbonated apatite and crystalline octacalcium phosphate-coated titanium discs. , 2005, Biomaterials.

[49]  Zuisei Kanno,et al.  Effects of mechanical strain on proliferation and differentiation of bone marrow stromal cell line ST2 , 2004, Journal of Bone and Mineral Metabolism.

[50]  F. Rozema,et al.  Cytotoxicity of poly(96L/4D-lactide): the influence of degradation and sterilization. , 2000, Biomaterials.

[51]  S. Mundlos,et al.  Cbfa1, a Candidate Gene for Cleidocranial Dysplasia Syndrome, Is Essential for Osteoblast Differentiation and Bone Development , 1997, Cell.

[52]  W. E. Brown,et al.  Crystal chemistry of octacalcium phosphate , 1981 .