Screening, isolation and identification of pectinas e producing bacterial strains from rotting fruits and determination of their pectinolytic acti vity

Naturally occurring polysaccharide pectin, the meth ylated ester of polygalacturonic acid, is very impo rtance in both scientific and commercial world due to its biodegradability. A lar ge group of pectinase enzymes causing breakdown of pectin polysaccharides of plants and fruit are used in industrial sector t o increase the yield and clarity of fruit juices. In this study, two bacterial strains were isolated using dilutions of 10 -4 and 10 -6 of rotten oranges. Isolated organisms were identif ied based on staining and biochemical tests. The pectinolytic activity was de termined using pectin containing minimal essential medium. The methodology applied was Kirby Bauer agar well diffusion method at the temperature is 35 ± 2 0 C. Based on Gram staining, spore staining and biochemical tests, two bacterial strains L ( Staphylococcus aureus ) and M ( Bacillus cereus ) were isolated and identified. Both strains showed different pectinolytic zones dependi ng on the concentration of inoculum and the largest pectinolytic zones of 25 mm was observed by both strains. These two strains are efficient and have potential to be implicated c ommercially to increase the clarity and quality of fruit juices.

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