DNA Polymerase (cid:100) Is Required for Human Mismatch Repair in Vitro *

HeLa nuclear extract was resolved into a depleted fraction incapable of supporting mismatch repair in vitro, and repair activity was restored upon the addition of a purified fraction isolated from HeLa cells by in vitrocomplementation assay. The highly enriched complementing activity copurified with a DNA polymerase, and the most pure fraction contained DNA polymerase δ but was free of detectable DNA polymerases α and ε. Calf thymus DNA polymerase δ also fully restored mismatch repair to the depleted extract, indicating DNA polymerase δ is required for mismatch repair in human cells. However, due to the presence of DNA polymerases α and ε in the depleted extract, potential involvement of one or both of these activities in the reaction cannot be excluded.

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