Application of enzyme-linked immunosorbent assay for the seroepizootiological survey of antibodies against porcine cytomegalovirus.

Enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against porcine cytomegalovirus (PCMV) was developed using the OF-1 strain of PCMV as an antigen. Results of the ELISA were compared to those of indirect fluorescent antibody (IFA) and serum neutralization (SN) tests. ELISA and IFA test were found to be extremely sensitive more than SN test. All of 11 tested sera were highly reactive in both ELISA and IFA test, but 6 of them were antibody-negative in the SN test. The retrospective survey of 436 fattening pig sera collected in Japan in 1981 showed that 433 (99.4%) of them were highly antibody-positive in ELISA.

[1]  H. Andersen.  Complement-fixing and virus-neutralizing antibodies in cytomegalovirus infection as measured against homologous and heterologous antigen. , 2009, Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology.

[2]  T. Tajima,et al.  Replication of porcine cytomegalovirus in the 19-PFT cell line. , 1992, The Journal of veterinary medical science.

[3]  A. Kaplan,et al.  Role of glycoproteins of pseudorabies virus in eliciting neutralizing antibodies. , 1986, Virology.

[4]  J. Shirai,et al.  A cytomegalovirus isolated from swine testicle cell culture. , 1985, Nihon juigaku zasshi. The Japanese journal of veterinary science.

[5]  A. Bouillant,et al.  Enzyme linked immunosorbent assay (ELISA) for the detection of antibodies to porcine cytomegalovirus. , 1982, Canadian journal of comparative medicine : Revue canadienne de medecine comparee.

[6]  T. Yoshikawa,et al.  Pathology of cytomegalic inclusion body disease in swine. , 1977, Nihon juigaku zasshi. The Japanese journal of veterinary science.

[7]  W. Plowright,et al.  Cytomegalovirus excretion in gnotobiotic pigs , 1976, Journal of Hygiene.

[8]  H. Kawamura.  Porcine Cytomegalovirus Infection , 1991 .