Expression of Arabidopsis response regulator homologs is induced by cytokinins and nitrate

We examined cytokinin and nitrate responsiveness in gene expression of five distinct response regulator homologs (ARR3–ARR7) in the leaves of nitrogen‐starved Arabidopsis plants. The transcripts accumulated after spraying the shoots with t‐zeatin. The induction of accumulation was highly specific for cytokinins. The transcripts also accumulated by supply of nitrate to the culture medium. These findings suggest that ARRs are involved in inorganic nitrogen signal transduction mediated by cytokinin as in the case of ZmCip1, a response regulator homolog recently identified in maize.

[1]  J. M. Horgan,et al.  Cytokinins and the Growth Responses of Seedlings of Betula pendula Roth. and Acer pseudoplatanus L. to Nitrogen and Phosphorus Deficiency , 1980 .

[2]  H. Saito,et al.  Two-component signal transducers and MAPK cascades. , 1997, Trends in biochemical sciences.

[3]  D. Phillips,et al.  Nitrogen stress regulates gene expression of enzymes in the flavonoid biosynthetic pathway of tomato [anthocyane] , 1995 .

[4]  Hsiao-Ching Yen,et al.  An Ethylene-Inducible Component of Signal Transduction Encoded by Never-ripe , 1995, Science.

[5]  H. Lambers,et al.  Cytokinin concentration in relation to mineral nutrition and benzyladenine treatment in Plantago major ssp. pleiosperma , 1989 .

[6]  J. S. Parkinson,et al.  Communication modules in bacterial signaling proteins. , 1992, Annual review of genetics.

[7]  H. Sakakibara,et al.  A response-regulator homologue possibly involved in nitrogen signal transduction mediated by cytokinin in maize. , 1998, The Plant journal : for cell and molecular biology.

[8]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[9]  I. Suzuki,et al.  Transcriptional and Posttranscriptional Regulation of Nitrogen-Responding Expression of Phosphoenolpyruvate Carboxylase Gene in Maize , 1994, Plant physiology.

[10]  E. Meyerowitz,et al.  Arabidopsis ethylene-response gene ETR1: similarity of product to two-component regulators. , 1993, Science.

[11]  R. W. Davis,et al.  Sequence and nitrate regulation of the Arabidopsis thaliana mRNA encoding nitrate reductase, a metalloflavoprotein with three functional domains. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[12]  S. Howell,et al.  Cytokinins and auxins control the expression of a gene in Nicotiana plumbaginifolia cells by feedback regulation. , 1992, The Plant cell.

[13]  H. Göring,et al.  Einfluß von Stickstoffmangel auf das K/Ca-Verhältnis und den Zytokiningehalt junger Kürbispflanzen , 1976 .

[14]  F. Ausubel,et al.  Transcriptional regulation of the Arabidopsis thaliana chalcone synthase gene , 1988, Molecular and cellular biology.

[15]  H. Goodman,et al.  Differential expression of the two Arabidopsis nitrate reductase genes. , 1991, Plant physiology.

[16]  E. Meyerowitz,et al.  Ethylene insensitivity conferred by Arabidopsis ERS gene. , 1995, Science.

[17]  F. Skoog,et al.  A revised medium for rapid growth and bio assays with tobacco tissue cultures , 1962 .

[18]  M. Hirai,et al.  Effects of sulfur nutrition on expression of the soybean seed storage protein genes in transgenic petunia. , 1992, Plant physiology.

[19]  K. Thimann,et al.  The relation between nitrogen deficiency and leaf senescence , 1984 .

[20]  T. Kakimoto.  CKI1, a Histidine Kinase Homolog Implicated in Cytokinin Signal Transduction , 1996, Science.

[21]  P. Gray,et al.  Use of aurintricarboxylic acid as an inhibitor of nucleases during nucleic acid isolation. , 1977, Nucleic acids research.

[22]  Ecker.  The ethylene signal transduction pathway in plants , 1995, Science.

[23]  R. Vierstra,et al.  Structure and evolution of genes encoding polyubiquitin and ubiquitin-like proteins in Arabidopsis thaliana ecotype Columbia. , 1995, Genetics.

[24]  J. Deikman,et al.  Induction of Anthocyanin Accumulation by Cytokinins in Arabidopsis thaliana , 1995, Plant physiology.

[25]  T. Mizuno,et al.  Response regulators implicated in His-to-Asp phosphotransfer signaling in Arabidopsis. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[26]  M. Simon,et al.  Signal transduction pathways involving protein phosphorylation in prokaryotes. , 1991, Annual review of biochemistry.