Enumeration of Escherichia coli in cooked and raw meats by ion mobility spectrometry.

A novel method for enumerating Escherichia coli in foods is described. It is based on the production of o-nitrophenol (ONP) from o-nitrophenyl-beta-D-glucuronide as detected by an ion mobility spectrometer. The time taken for the detection of ONP in a food sample was inversely proportional to the logarithm of the initial E. coli population. Escherichia coli could be detected at a level of 10 g-1 within 9 h.

[1]  I. Ogden,et al.  An evaluation of fluorogenic and chromogenic assays for the direct enumeration of Escherichia coli , 1991 .

[2]  A. Snyder,et al.  Detection of bacteria by ion mobility spectrometry. , 1991, Analytical chemistry.

[3]  M. Clifford,et al.  Colorimetric enumeration of Escherichia coli based on beta-glucuronidase activity , 1990, Applied and environmental microbiology.

[4]  P. Entis.  Hydrophobic grid membrane filter/MUG method for total coliform and Escherichia coli enumeration in foods: collaborative study. , 1989, Journal - Association of Official Analytical Chemists.

[5]  E. W. Frampton,et al.  Evaluation of the β-Glucuronidase Substrate 5-Bromo-4-Chloro-3-Indolyl-β-D-Glucuronide (X-GLUC) in a 24-Hour Direct Plating Method for Escherichia coli. , 1988, Journal of food protection.

[6]  J. Pérez,et al.  Evaluation of a commercial beta-glucuronidase test for the rapid and economical identification of Escherichia coli. , 1986, The Journal of applied bacteriology.

[7]  E. Todd,et al.  Method to Isolate Escherichia coli O157:H7 from Food. , 1986, Journal of food protection.

[8]  M. Doyle,et al.  Survival and growth characteristics of Escherichia coli associated with hemorrhagic colitis , 1984, Applied and environmental microbiology.

[9]  P. Feng,et al.  Fluorogenic assays for immediate confirmation of Escherichia coli , 1982, Applied and environmental microbiology.

[10]  N. D. Cowell,et al.  Microbiological techniques--some statistical aspects. , 1969, Journal of the science of food and agriculture.