Human immunodeficiency virus‐associated malignant lymphoma in eastern Denmark diagnosed from 1990 to 1996: clinical features, histopathology, and association with Epstein‐Barr virus and human herpesvirus‐8

Abstract: The clinicopathological features of human immunodeficiency virus (HIV)‐associated lymphoma were investigated in a retrospective study of 85 adult patients in eastern Denmark diagnosed during the period 1990–1996. The possible pathogenetic role of Epstein‐Barr virus (EBV) and human herpesvirus 8 (HHV‐8) in these tumours was also studied. Seventy patients (82%) presented with extranodal disease and 26 (31%) had CNS involvement at diagnosis. Diffuse large cell B‐cell lymphoma was the most frequent histological subtype, comprising 65 of 79 cases available for microscopic re‐evaluation (82%) and including 20 of 23 evaluable patients with CNS lymphoma (87%). EBV RNA was demonstrated by in situ hybridization in 51 of 65 evaluable tumours (79%) and in 14 of 16 cases (88%) with CNS‐lymphoma. Three cases showed a T‐cell phenotype. The presence of HHV‐8 DNA was analysed by PCR in 32 cases. A strong band consistent with tumour cell infection was detected in only one case, weaker bands being seen in 4 cases. None of these patients had primary effusion lymphomas. In conclusion, Danish AIDS‐related lymphomas are of predominantly high‐grade B‐cell type with extranodal localization and atypical presentation. Our results provide further evidence that EBV plays a major role in the pathogenesis of large cell AIDS‐related lymphoma, whereas HHV‐8 does not appear to contribute significantly to the development of solid lymphomas in this group of patients.

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