Comparison of different pretreatment methods for enriching hydrogen-producing bacteria from digested sludge

Abstract The pretreatment of digested sludge by five methods (by acid, base, heat-shock, aeration and chloroform, respectively) was conducted in batch tests to evaluate and compare their suitability in the enrichment of hydrogen-producing bacteria. The experimental results showed that, at 35 °C and initial pH 7.0, the hydrogen yields of the pretreated digested sludge were higher in comparison with the control test during the fermentative hydrogen production using glucose as the substrate. In all tests, for fermentative hydrogen production from glucose, the digested sludge pretreated by heat-shock could obtain the maximal hydrogen production potential, maximum hydrogen production rate, hydrogen yield, substrate degradation efficiency and biomass concentration, which were 215.4 mL, 120.4 mL/h, 221.5 mL/g glucose, 97.2% and 2739 mg/L, respectively. The final pH values in the liquid after fermentative hydrogen production in all tests were between 3.4 and 4.1. Heat-shock is an easy and practical pretreatment method for enriching hydrogen-producing bacteria from digested sludge.

[1]  Yuansong Wei,et al.  Enhanced biohydrogen production from sewage sludge with alkaline pretreatment. , 2004, Environmental science & technology.

[2]  Heguang Zhu,et al.  Evaluation of alternative methods of preparing hydrogen producing seeds from digested wastewater sludge , 2006 .

[3]  J. Lay,et al.  Biohydrogen generation by mesophilic anaerobic fermentation of microcrystalline cellulose. , 2001, Biotechnology and bioengineering.

[4]  Sangeun Oh,et al.  The relative effectiveness of pH control and heat treatment for enhancing biohydrogen gas production. , 2003, Environmental science & technology.

[5]  Jianlong Wang,et al.  Effect of Fe2+ concentration on fermentative hydrogen production by mixed cultures , 2008 .

[6]  Chyi-How Lay,et al.  Effects of carbonate and phosphate concentrations on hydrogen production using anaerobic sewage sludge microflora , 2004 .

[7]  N. Ren,et al.  Assessing optimal fermentation type for bio-hydrogen production in continuous-flow acidogenic reactors. , 2007, Bioresource technology.

[8]  Sheng-Shung Cheng,et al.  Behavioral study on hydrogen fermentation reactor installed with silicone rubber membrane , 2002 .

[9]  Haijun Yang,et al.  Effect of ferrous iron concentration on anaerobic bio-hydrogen production from soluble starch , 2006 .

[10]  Jianquan Shen,et al.  Hydrogen production in batch culture of mixed bacteria with sucrose under different iron concentrations , 2005 .

[11]  Chin-Chao Chen,et al.  Acid–base enrichment enhances anaerobic hydrogen production process , 2001, Applied Microbiology and Biotechnology.

[12]  B. Logan,et al.  Biotechnological Products and Process Engineering H 2 -producing Bacterial Communities from a Heat-treated Soil Inoculum , 2022 .

[13]  Michael Kornaros,et al.  Hydrogen production from sugars and sweet sorghum biomass using Ruminococcus albus , 2008 .

[14]  P. N. Sarma,et al.  Effect of various pretreatment methods on anaerobic mixed microflora to enhance biohydrogen production utilizing dairy wastewater as substrate. , 2008, Bioresource technology.

[15]  Bo Hu,et al.  Pretreatment of methanogenic granules for immobilized hydrogen fermentation , 2007 .

[16]  Chiu-Yue Lin,et al.  Carbon/nitrogen-ratio effect on fermentative hydrogen production by mixed microflora , 2004 .

[17]  C. Y. Lin,et al.  Anaerobic Hydrogen Production from Sucrose Using an Acid‐Enriched Sewage Sludge Microflora , 2004 .

[18]  Sang-Eun Oh,et al.  Biological hydrogen production measured in batch anaerobic respirometers. , 2002, Environmental science & technology.

[19]  Han-Qing Yu,et al.  Inhibitory effects of butyrate on biological hydrogen production with mixed anaerobic cultures. , 2005, Journal of environmental management.

[20]  D. Cheong,et al.  Bacterial stress enrichment enhances anaerobic hydrogen production in cattle manure sludge , 2006, Applied Microbiology and Biotechnology.

[21]  Chiu-Yue Lin,et al.  Biohydrogen production using an up-flow anaerobic sludge blanket reactor , 2004 .

[22]  J. Lay,et al.  Feasibility of biological hydrogen production from organic fraction of municipal solid waste , 1999 .

[23]  Chiu-Yue Lin,et al.  A nutrient formulation for fermentative hydrogen production using anaerobic sewage sludge microflora , 2005 .

[24]  Herbert H. P. Fang,et al.  Fermentative Hydrogen Production From Wastewater and Solid Wastes by Mixed Cultures , 2007 .

[25]  Gaosheng Zhang,et al.  Optimization of initial substrate and pH levels for germination of sporing hydrogen-producing anaerobes in cow dung compost. , 2004, Bioresource technology.

[26]  G. L. Miller.  Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar , 1959 .

[27]  J C Huang,et al.  Ethanol-type fermentation from carbohydrate in high rate acidogenic reactor. , 1997, Biotechnology and bioengineering.

[28]  Peter Dürre,et al.  Production and Utilization of Ethanol by the Homoacetogen Acetobacterium woodii , 1989, Applied and environmental microbiology.

[29]  Lei Wang,et al.  Avoiding propionic acid accumulation in the anaerobic process for biohydrogen production , 2006 .

[30]  Han-Qing Yu,et al.  Biological hydrogen production by anaerobic sludge at various temperatures , 2006 .

[31]  Richard Sparling,et al.  Hydrogen production by Clostridium thermocellum 27405 from cellulosic biomass substrates , 2006 .

[32]  Gang Wang,et al.  Evaluation of three methods for enriching H2-producing cultures from anaerobic sludge , 2007 .

[33]  Chiu-Yue Lin,et al.  Biohydrogen production from sucrose using base-enriched anaerobic mixed microflora , 2006 .

[34]  Ahmadun Fakhru’l-Razi,et al.  Biological production of hydrogen from glucose by natural anaerobic microflora , 2004 .

[35]  A. E. Greenberg,et al.  Standard methods for the examination of water and wastewater : supplement to the sixteenth edition , 1988 .