ISOENZYMES AND ACTIVITY OF LACCASES PRODUCED BY Pleurotus ostreatus GROWN AT DIFFERENT TEMPERATURES

Pleurotus ostreatus produces laccase isoenzymes depending n the con itions in which it grows, it has been suggested that the presence of inducers, the pH of the culture medium as well as the type and concentration of carbon and nitrogen sources promote the production of these isoenzymes, however, the effect of temperature of Pleurotus ostreatus growth on its production of laccase isoenzymes has not been evaluated. In this study, Pleurotus ostreatus was grown in solid-state fermentation at different temperatures (10, 15, 20, 25, 30 and 35 °C) and the activity and isoenzymes number of laccases produced in each bioprocess was determined. At 10 and 35 °C there was no growth of the fungus. The activity of laccases was different in each fermentation, being at 25 oC where the highest value was observed (20000 U L−1). The laccase isoe zymes pattern was also differe t depending on the incubation temperature of the fungus, it was observed in some times of the culture, 4 isoenzymes in the fermentations incubated at 15 and 20 oC, 3 isoenzymes at 25 oC and 2 isoenzymes at 30 oC. These results show that the growth temperature of Pleurotus ostreatus modifies its activity profiles and its isoenzyme patterns of laccases.

[1]  S. Furlan,et al.  STUDY OF THE REMOVAL OF BISPHENOL-A BY Pleurotus sajor-caju CRUDE ENZYME BROTH , 2018, Revista Mexicana de Ingeniería Química.

[2]  L. Sánchez-Minutti,et al.  Biodegradation of azo dyes by Pleurotus ostreatus , 2018 .

[3]  Muhammad Amjad Ali,et al.  EFFECTS OF DIFFERENT CULTURE MEDIA, TEMPERATURE AND pH LEVELS ON THE GROWTH OF WILD AND EXOTIC PLEUROTUS SPECIES , 2016 .

[4]  G. Díaz-Godínez,et al.  CHARACTERIZATION OF PRODUCTION OF LACCASES, CELLULASES AND XYLANASES OF Pleurotus ostreaus GROWN ON SOLID-STATE FERMENTATION USING AN INERT SUPPORT , 2016 .

[5]  Hà Thị Hòa,et al.  The Effects of Temperature and Nutritional Conditions on Mycelium Growth of Two Oyster Mushrooms (Pleurotus ostreatus and Pleurotus cystidiosus) , 2015, Mycobiology.

[6]  Claudia Patricia Soto-Arroyave,et al.  Cinética del crecimiento de Pleurotus ostreatus en la etapa de producción del cuerpo fructífero , 2015 .

[7]  C. Sánchez,et al.  DESCRIPTION OF A LACCASE GENE FROM PLEUROTUS OSTREATUS EXPRESSED UNDER SUBMERGED FERMENTATION CONDITIONS , 2012 .

[8]  Vincenzo Lettera,et al.  Transcriptional analysis of Pleurotus ostreatus laccase genes , 2012, Applied Microbiology and Biotechnology.

[9]  Hexiang Wang,et al.  A laccase with anti-proliferative activity against tumor cells from a white root fungus Abortiporus biennis , 2011 .

[10]  V. Faraco,et al.  Induction and Transcriptional Regulation of Laccases in Fungi , 2011, Current genomics.

[11]  C. Sánchez,et al.  CHARACTERIZATION OF THE GROWTH AND LACCASE ACTIVITY OF STRAINS OF PLEUROTUS OSTREATUS IN SUBMERGED FERMENTATION , 2011 .

[12]  S. Lele,et al.  Bioprocessing of laccase produced by submerged culture of Ganoderma sp. WR-1 , 2010 .

[13]  R. Choi,et al.  An induction effect of heat shock on the transcript of globular acetylcholinesterase in NG108-15 cells. , 2010, Chemico-biological interactions.

[14]  R. S. St. Leger,et al.  A laccase exclusively expressed by Metarhizium anisopliae during isotropic growth is involved in pigmentation, tolerance to abiotic stresses and virulence. , 2010, Fungal genetics and biology : FG & B.

[15]  C. Sánchez,et al.  Growth and laccase production by Pleurotus ostreatus in submerged and solid-state fermentation , 2008, Applied Microbiology and Biotechnology.

[16]  P. Widsten,et al.  Laccase applications in the forest products industry : A review , 2008 .

[17]  P. Baldrian,et al.  Temperature affects the production, activity and stability of ligninolytic enzymes inPleurotus ostreatus andTrametes versicolor , 2008, Folia Microbiologica.

[18]  Ravichandra Potumarthi,et al.  Enhancement of gentamicin production by mutagenesis and non-nutritional stress conditions in Micromonospora echinospora , 2007 .

[19]  Helen Treichel,et al.  Production of inulinase by solid-state fermentation: effect of process parameters on production and preliminary characterization of enzyme preparations , 2007, Bioprocess and biosystems engineering.

[20]  C. Perry Chou,et al.  Engineering cell physiology to enhance recombinant protein production in Escherichia coli , 2007, Applied Microbiology and Biotechnology.

[21]  C. Soccol,et al.  Effect of stress on growth, pigment production and morphology of Monascus sp. in solid cultures , 2007, Journal of basic microbiology.

[22]  M. Berovič,et al.  Heat shock on Saccharomyces cerevisiae inoculum increases glycerol production in wine fermentation , 2007, Biotechnology Letters.

[23]  Davood Zareh,et al.  Production of xylanase by Trichoderma longibrachiatum on a mixture of wheat bran and wheat straw: Optimization of culture condition by Taguchi method , 2007 .

[24]  P. Baldrian.  Fungal laccases - occurrence and properties. , 2006, FEMS microbiology reviews.

[25]  D. Choi,et al.  Studies on cultivation and biological activities ofPleurotus nebrodensis inzenga , 2006 .

[26]  D. Madamwar,et al.  Xylanase production by a newly isolated Aspergillus foetidus strain and its characterization , 2005 .

[27]  T. Ng,et al.  Peptides and proteins from fungi , 2004, Peptides.

[28]  G. Sannia,et al.  Stability and activity of a phenol oxidase from the ligninolytic fungus Pleurotus ostreatus , 1993, Applied Microbiology and Biotechnology.

[29]  K. Langfelder,et al.  Biosynthesis of fungal melanins and their importance for human pathogenic fungi. , 2003, Fungal genetics and biology : FG & B.

[30]  Nelson Durán,et al.  Applications of laccases and tyrosinases (phenoloxidases) immobilized on different supports: a review , 2002 .

[31]  M. Hofrichter.  Review: lignin conversion by manganese peroxidase (MnP) , 2002 .

[32]  A. Matuszewska,et al.  Fungal laccase: properties and activity on lignin , 2001, Journal of basic microbiology.

[33]  G. Viniegra-González,et al.  Exopectinases produced by Aspergillus niger in solid-state and submerged fermentation: a comparative study , 2001, Journal of Industrial Microbiology and Biotechnology.

[34]  L. Jecu.  Solid state fermentation of agricultural wastes for endoglucanase production , 2000 .

[35]  A. Matuszewska,et al.  Biodegradation of lignin by white rot fungi. , 1999, Fungal genetics and biology : FG & B.

[36]  C. Thurston.  The structure and function of fungal laccases , 1994 .

[37]  T. Higuchi,et al.  Biodegradation of Lignin , 1981 .